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Statements

Subject Item
n2:RIV%2F49608851%3A_____%2F12%3A%230000580%21RIV13-MZE-49608851
rdf:type
skos:Concept n5:Vysledek
dcterms:description
The aim of the study was to test commercial extenders used for short-term and long-term sperm preservation. Semen was collected in the reproduction season, i.e. from June to December. The ejaculates were obtained from single services and the routine analysis of the semen was performed immediately after the collection. The examination included semen volume, colour and texture, sperm concentration and motility, ejaculate turbulence and percentage of sperm with abnormal morphology. The semen was diluted with an extender in the ratio of 1:4. The processed semen was transported in an insulated container at 16–18 °C to the laboratory and stored in a stationary thermostat under the same temperature. Sperm motility tests were performed 24, 48, 72 and 96 hours after the placement in to thermostat. Ejaculates diluted with Ovipro, Optidyl, Triladyl and Andromed CSS gave very good results of viability (81.23 %–83.41 %) after 24 hours of storage. After 48 hours, Ovipro, Andromed, Optidyl and Triladyl gave values above 75 %. The Triladyl extender proved to be a good stabilizing agent, showing consistent results during a long-term storage. It was chosen as a control one for overall assessment. Other preservation media did not show any improving or worsening effects. The extender Ovipro showed a high motility effect in the first 48 hours only, and hence it appears to be the best solution for the short-term preservation. The aim of the study was to test commercial extenders used for short-term and long-term sperm preservation. Semen was collected in the reproduction season, i.e. from June to December. The ejaculates were obtained from single services and the routine analysis of the semen was performed immediately after the collection. The examination included semen volume, colour and texture, sperm concentration and motility, ejaculate turbulence and percentage of sperm with abnormal morphology. The semen was diluted with an extender in the ratio of 1:4. The processed semen was transported in an insulated container at 16–18 °C to the laboratory and stored in a stationary thermostat under the same temperature. Sperm motility tests were performed 24, 48, 72 and 96 hours after the placement in to thermostat. Ejaculates diluted with Ovipro, Optidyl, Triladyl and Andromed CSS gave very good results of viability (81.23 %–83.41 %) after 24 hours of storage. After 48 hours, Ovipro, Andromed, Optidyl and Triladyl gave values above 75 %. The Triladyl extender proved to be a good stabilizing agent, showing consistent results during a long-term storage. It was chosen as a control one for overall assessment. Other preservation media did not show any improving or worsening effects. The extender Ovipro showed a high motility effect in the first 48 hours only, and hence it appears to be the best solution for the short-term preservation.
dcterms:title
Effect of different extenders on ram sperm traits during storage Effect of different extenders on ram sperm traits during storage
skos:prefLabel
Effect of different extenders on ram sperm traits during storage Effect of different extenders on ram sperm traits during storage
skos:notation
RIV/49608851:_____/12:#0000580!RIV13-MZE-49608851
n5:predkladatel
n6:ico%3A49608851
n3:aktivita
n16:P
n3:aktivity
P(QH81324)
n3:cisloPeriodika
6
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n17:9608230
n3:druhVysledku
n14:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
132993
n3:idVysledku
RIV/49608851:_____/12:#0000580
n3:jazykVysledku
n4:eng
n3:klicovaSlova
ram; sperm activity; extender
n3:klicoveSlovo
n10:extender n10:ram n10:sperm%20activity
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[346C2271BB55]
n3:nazevZdroje
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
n3:obor
n18:GG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
5
n3:projekt
n13:QH81324
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
LX
n3:tvurceVysledku
Vejnar, Jan Hegedüšová, Zdeňka Louda, František Štolc, Ladislav Čunát, Libor
s:issn
1211-8516
s:numberOfPages
5