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Statements

Subject Item
n2:RIV%2F49608851%3A_____%2F11%3A%230000502%21RIV12-MZE-49608851
rdf:type
skos:Concept n12:Vysledek
dcterms:description
The aim of the study was to compare the use of rapid thermoresistance test (sperm activity), CASA (sperm motility) analysis and morphology evaluation for predicting fertility of frozen-thawed ram semen following AI. The evaluation of the frozen-thawed semen from East-Friesian (EF) and Lacaune (Lc) breed rams showed significant (P < 0.001) differences in the spermatozoa activity at the different time points of the thermoresistance test (30 and 60 minutes) after thawing between these rams. The spermatozoa activity of EF semen after 15, 30 and 60 min of incubation was maintained at the same level (30 %). However, the sperm activity of Lc rams decreased from 25 to 10 %. In accordance with the decrease of Lc sperm activity, morphological evaluation showed higher percentage of malformations in the head, midpeace and tail regions of Lc sperm. These results are in concert with CASA analysis of sperm motility. A higher total and progressive motility at 30 and 120 min after thawing was measured in sperm from EF ram (P < 0.001) compared to the sperm from Lc ram. The differences detected in the motility parameters of frozen-thawed semen from two ram breeds were reflected in the pregnancy (12.24 and 28.00 %) and lambing rate (0.0 and 56.00 %) for EF and Lc respectively. The aim of the study was to compare the use of rapid thermoresistance test (sperm activity), CASA (sperm motility) analysis and morphology evaluation for predicting fertility of frozen-thawed ram semen following AI. The evaluation of the frozen-thawed semen from East-Friesian (EF) and Lacaune (Lc) breed rams showed significant (P < 0.001) differences in the spermatozoa activity at the different time points of the thermoresistance test (30 and 60 minutes) after thawing between these rams. The spermatozoa activity of EF semen after 15, 30 and 60 min of incubation was maintained at the same level (30 %). However, the sperm activity of Lc rams decreased from 25 to 10 %. In accordance with the decrease of Lc sperm activity, morphological evaluation showed higher percentage of malformations in the head, midpeace and tail regions of Lc sperm. These results are in concert with CASA analysis of sperm motility. A higher total and progressive motility at 30 and 120 min after thawing was measured in sperm from EF ram (P < 0.001) compared to the sperm from Lc ram. The differences detected in the motility parameters of frozen-thawed semen from two ram breeds were reflected in the pregnancy (12.24 and 28.00 %) and lambing rate (0.0 and 56.00 %) for EF and Lc respectively.
dcterms:title
Interrelationship between motility and in vivo fertility of ram frozen-thawed semen Interrelationship between motility and in vivo fertility of ram frozen-thawed semen
skos:prefLabel
Interrelationship between motility and in vivo fertility of ram frozen-thawed semen Interrelationship between motility and in vivo fertility of ram frozen-thawed semen
skos:notation
RIV/49608851:_____/11:#0000502!RIV12-MZE-49608851
n12:predkladatel
n15:ico%3A49608851
n4:aktivita
n13:P
n4:aktivity
P(QH81324)
n4:cisloPeriodika
3
n4:dodaniDat
n10:2012
n4:domaciTvurceVysledku
n5:8204942 n5:3511316 n5:9608230
n4:druhVysledku
n14:J
n4:duvernostUdaju
n7:S
n4:entitaPredkladatele
n18:predkladatel
n4:idSjednocenehoVysledku
205520
n4:idVysledku
RIV/49608851:_____/11:#0000502
n4:jazykVysledku
n16:eng
n4:klicovaSlova
ram; semen; sperm activity; motility; pregnancy
n4:klicoveSlovo
n8:sperm%20activity n8:pregnancy n8:semen n8:motility n8:ram
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[8E0798210C42]
n4:nazevZdroje
Výzkum v chovu skotu
n4:obor
n6:GG
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
6
n4:projekt
n17:QH81324
n4:rokUplatneniVysledku
n10:2011
n4:svazekPeriodika
LIII
n4:tvurceVysledku
Chrenek, Petr Apolen, Dušan Špaleková, Eliška Kubovičová, Elena Makarevič, Alexander Hegedüšová, Zdeňka
s:issn
0139-7265
s:numberOfPages
7