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Statements

Subject Item
n2:RIV%2F49608851%3A_____%2F11%3A%230000495%21RIV12-MZE-49608851
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100,200 or 400 ng/ml). Following 72-96 h storage at 4 degrees C, the spermatozoa were stained for a plasma membrane integrity (PNA-FITC), membrane phosphatidylserine (PS) translocation (annexin V-Fluos) and apoptosis (Yo-Pro-1), and analyzed by fluorescent microscopy. Sperm motility was measured using computer-assisted sperm analysis (CASA) and sperm fertilizing ability was tested using in vitro penetration/fertilization test on bovine prematured oocytes. EGF increased sperm motility at all doses tested, decreased the proportion of spermatozoa with damaged plasma membrane (at 200 or 400 ng/ml), and decreased the proportion of apoptotic (Yo-Pro-1) spermatozoa when given at 200 or 400 (but not 100) ng/ml. The proportion of spermatozoa with PS translocations (8.5%) was affected by neither of the EGF concentrations tested. However, fertilizing ability of ram sperm in the in vitro test was not improved by EGF (200 ng/ml). In summary, EGF when given at higher concentrations improved sperm viability and motility after cooling storage, but these effects were not reflected in sperm fertilizing ability in vitro. The aim of the study was to examine the effects of epidermal growth factor (EGF) on ram sperm traits following hypothermic storage. Fresh ram ejaculates were diluted in Triladyl extender, pooled and divided into groups according to EGF doses added (0, 100,200 or 400 ng/ml). Following 72-96 h storage at 4 degrees C, the spermatozoa were stained for a plasma membrane integrity (PNA-FITC), membrane phosphatidylserine (PS) translocation (annexin V-Fluos) and apoptosis (Yo-Pro-1), and analyzed by fluorescent microscopy. Sperm motility was measured using computer-assisted sperm analysis (CASA) and sperm fertilizing ability was tested using in vitro penetration/fertilization test on bovine prematured oocytes. EGF increased sperm motility at all doses tested, decreased the proportion of spermatozoa with damaged plasma membrane (at 200 or 400 ng/ml), and decreased the proportion of apoptotic (Yo-Pro-1) spermatozoa when given at 200 or 400 (but not 100) ng/ml. The proportion of spermatozoa with PS translocations (8.5%) was affected by neither of the EGF concentrations tested. However, fertilizing ability of ram sperm in the in vitro test was not improved by EGF (200 ng/ml). In summary, EGF when given at higher concentrations improved sperm viability and motility after cooling storage, but these effects were not reflected in sperm fertilizing ability in vitro.
dcterms:title
Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor
skos:prefLabel
Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor Functional characteristics of ram cooling-stored spermatozoa under the influence of epidermal growth factor
skos:notation
RIV/49608851:_____/11:#0000495!RIV12-MZE-49608851
n11:predkladatel
n15:ico%3A49608851
n3:aktivita
n5:P n5:Z
n3:aktivity
P(QH81324), Z(MSM2678846201)
n3:cisloPeriodika
SI
n3:dodaniDat
n9:2012
n3:domaciTvurceVysledku
n20:9608230
n3:druhVysledku
n18:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
200520
n3:idVysledku
RIV/49608851:_____/11:#0000495
n3:jazykVysledku
n19:eng
n3:klicovaSlova
ram; sperm; viability; motility; fertilizing ability
n3:klicoveSlovo
n4:ram n4:motility n4:fertilizing%20ability n4:viability n4:sperm
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[5ECB7A6C57DB]
n3:nazevZdroje
GENERAL PHYSIOLOGY AND BIOPHYSICS
n3:obor
n7:GG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
6
n3:projekt
n17:QH81324
n3:rokUplatneniVysledku
n9:2011
n3:svazekPeriodika
30
n3:tvurceVysledku
Špaleková, Eliška Kubovičová, Elena Makarevič, Alexander Lukáč, Norbert Olexíková, Lucia Hegedüšová, Zdeňka
n3:wos
000293112300005
n3:zamer
n16:MSM2678846201
s:issn
0231-5882
s:numberOfPages
8
n12:doi
10.4149/gpb_2011_SI1_36