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Statements

Subject Item
n2:RIV%2F49608851%3A_____%2F07%3A8P009145%21RIV08-MZE-49608851
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Cílem práce bylo prověřit zda vystavení bovinních embryí vlivu viru BHV-1 ovlivní jejich další vývoj a vyvolá změny v ultrastruktuře buněčných organel; jestli zóna pelucida je bariérou, která může ochránit embryo před infekcí a zda promytí embrya v trypsinu je ochranou před penetrací viru do embryí a jejich poškození.Na základě nálezů je možno konstatovat, že infekce virem BHV-1 umožňuje preimplantační vývoj bovinních embryí in vitro a proto zóna pelucida není dostatečnou bariérou proti virovým poškozením, jestliže embrya nejsou ošetřena promytím v trypsinu. The aim of the our study was to examine whether: the exposure of bovine embryos to the BHV-1 virus in vitro can compromise their further developmental and alter the ultrastructural morphology of cellular organelles; whether the zona pellucida /(ZP) can be a barrier protecting embryos against infection; and whether washing with typsin after exposure can prevent virus penetration inside the embryo and subsequent virus-induced damages. Taken together these findings may be suggested that BHV-1 infection compromises preimplantation development of bovine embryos in vitro and therefore the ZP may not be enough on its own to prevent virus-induced damage, unless it is not accompanied with trypsin washing. The aim of the our study was to examine whether: the exposure of bovine embryos to the BHV-1 virus in vitro can compromise their further developmental and alter the ultrastructural morphology of cellular organelles; whether the zona pellucida /(ZP) can be a barrier protecting embryos against infection; and whether washing with typsin after exposure can prevent virus penetration inside the embryo and subsequent virus-induced damages. Taken together these findings may be suggested that BHV-1 infection compromises preimplantation development of bovine embryos in vitro and therefore the ZP may not be enough on its own to prevent virus-induced damage, unless it is not accompanied with trypsin washing.
dcterms:title
Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies. Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies. Vývoj a viabilita bovinních preimplantačních embryí po infekci bovinním herpesvirem-1:immunocytochemické a ultramorfologické studie.
skos:prefLabel
Vývoj a viabilita bovinních preimplantačních embryí po infekci bovinním herpesvirem-1:immunocytochemické a ultramorfologické studie. Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies. Development and viability of bovine preimplantation embryos after the in vitro infection with bovine herpesvirus-1:immunocytochemical and ultrastructural studies.
skos:notation
RIV/49608851:_____/07:8P009145!RIV08-MZE-49608851
n3:strany
307-315
n3:aktivita
n12:P
n3:aktivity
P(1B44034)
n3:cisloPeriodika
4
n3:dodaniDat
n10:2008
n3:domaciTvurceVysledku
n5:7976089 n5:9717412 n5:3511316 n5:9653287 n5:8204942
n3:druhVysledku
n9:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
416807
n3:idVysledku
RIV/49608851:_____/07:8P009145
n3:jazykVysledku
n14:eng
n3:klicovaSlova
bovine herpesvirus-1; embryo; ultrastructure; trypsin washing
n3:klicoveSlovo
n8:bovine%20herpesvirus-1 n8:trypsin%20washing n8:ultrastructure n8:embryo
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[3DACAB0C600A]
n3:nazevZdroje
Zygote
n3:obor
n15:EI
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n13:1B44034
n3:rokUplatneniVysledku
n10:2007
n3:svazekPeriodika
15
n3:tvurceVysledku
Slezáková, Milena Louda, František Kubovičová, Elena Pivko, Juraj Makarevich, Alexandr Chrenek, P.
s:issn
0967-1994
s:numberOfPages
9