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Statements

Subject Item
n2:RIV%2F48392952%3A_____%2F13%3A%230000264%21RIV14-MSM-48392952
rdf:type
n12:Vysledek skos:Concept
dcterms:description
The capacity of most flax (Linum usitatissimum L.) cultivars to accumulate cadmium (Cd) brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. To investigate whether overproduction of an efficient heterologous Cd-binding peptide may enhance the phytoextraction capacity of L. usitatissimum, breeding line AGT 917 was engineered to constitutively express genetic fusion of α-domain of mammalian metallothionein 1a (αMT1a) and β-glucuronidase gus gene under the control of CaMV 35S promoter. To this end, an improved transformation protocol was developed, which involved 10-min incubation of hypocotyl segments with partially removed epidermis with Agrobacterium suspension in the presence of 100 mg.l-1 acetosyringone and 200 mg.l-1 cellulase and which increased transformation efficiency 1.6-fold as compared to agroinfection without combined acetosyringone and cellulase treatment. When tested in soils amended with Cd added at concentrations of 20 and 360 mg kg-1, the mature αMT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing αMT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of αMT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l-1, providing that αMT1a::gus confers higher Cd tolerance to engineered plant. Thus, overproduction of metal-binding peptides appears to be viable strategy for production of L. usitatissimum with improved phytoremediation capacity. The capacity of most flax (Linum usitatissimum L.) cultivars to accumulate cadmium (Cd) brought an interest in their use in phytoextraction of soil Cd to aboveground biomass. To investigate whether overproduction of an efficient heterologous Cd-binding peptide may enhance the phytoextraction capacity of L. usitatissimum, breeding line AGT 917 was engineered to constitutively express genetic fusion of α-domain of mammalian metallothionein 1a (αMT1a) and β-glucuronidase gus gene under the control of CaMV 35S promoter. To this end, an improved transformation protocol was developed, which involved 10-min incubation of hypocotyl segments with partially removed epidermis with Agrobacterium suspension in the presence of 100 mg.l-1 acetosyringone and 200 mg.l-1 cellulase and which increased transformation efficiency 1.6-fold as compared to agroinfection without combined acetosyringone and cellulase treatment. When tested in soils amended with Cd added at concentrations of 20 and 360 mg kg-1, the mature αMT1a::gus plants accumulated more Cd than parental AGT 917: the stem Cd concentrations in the best performing αMT1/2 line were 3.3- and 1.9-fold higher, respectively. Moreover, hypocotyl explants of αMT1/2 line showed 1.7-fold higher biomass than those of AGT 917 on media containing 15 mg Cd l-1, providing that αMT1a::gus confers higher Cd tolerance to engineered plant. Thus, overproduction of metal-binding peptides appears to be viable strategy for production of L. usitatissimum with improved phytoremediation capacity.
dcterms:title
Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein
skos:prefLabel
Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein Enhanced accumulation of cadmium in Linum usitatissimum L. plants due to overproduction of metallothionein alfa-domain as a fusion to beta-glucuronidase protein
skos:notation
RIV/48392952:_____/13:#0000264!RIV14-MSM-48392952
n12:predkladatel
n15:ico%3A48392952
n3:aktivita
n14:P n14:I
n3:aktivity
I, P(1M06030)
n3:cisloPeriodika
3
n3:dodaniDat
n9:2014
n3:domaciTvurceVysledku
n11:1697781 n11:4390334 n11:6371345 n11:1834118 n11:7711700 n11:4071107 n11:6246109
n3:druhVysledku
n16:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n5:predkladatel
n3:idSjednocenehoVysledku
72918
n3:idVysledku
RIV/48392952:_____/13:#0000264
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Linum usitatissimum L.; cadmium; flax; linseed; metallothionein; phytoextraction
n3:klicoveSlovo
n7:cadmium n7:Linum%20usitatissimum%20L. n7:flax n7:phytoextraction n7:linseed n7:metallothionein
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[B91FCA5C278B]
n3:nazevZdroje
Plant Cell Tissue and Organ Culture
n3:obor
n18:EI
n3:pocetDomacichTvurcuVysledku
7
n3:pocetTvurcuVysledku
8
n3:projekt
n8:1M06030
n3:rokUplatneniVysledku
n9:2013
n3:svazekPeriodika
112
n3:tvurceVysledku
Horáček, Jiří Větrovcová, Martina Kotrba, Pavel Smýkal, Petr Griga, Miroslav Smýkalová, Iva Vrbová, Miroslava Švábová, Lenka
n3:wos
000315277300006
s:issn
0167-6857
s:numberOfPages
9
n17:doi
10.1007/s11240-012-0239-1