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Statements

Subject Item
n2:RIV%2F47813644%3A_____%2F07%3A8P009912%21RIV08-MZE-47813644
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Restorer lines of Ogu-INRA hybrid system with RfRf gene and low glucosinolates content in seed were selected. On the begining selection of the restorer lines were carried out according to GSL content. Following selection was aimed at restoring ability in F1 generation after pollination of sterile CMS component. Total 284 restorer lines were tested according their restoring ability. The 65 restorer lines (i.e. 22.89 %) with restoring ability above 90 % were founded. The 45 restorer lines (i.e. 15.84 %) had also GSL content below 18 µmol/g seed. Molecular method using of SCAR marker SG34 was optimallized and successfuly used for markering of Rf restorer gene. Check test evaluation on 50 random selected restorer plants confirmed accuracy of detection dominant Rf gene using these molecular marker. Because of impossibility for recognize dominant homozygous and heterozygous forms of the Rf gene using of fertility restoring test according to fertile flowers in F1 generation in addition to molecular methods Restorer lines of Ogu-INRA hybrid system with RfRf gene and low glucosinolates content in seed were selected. On the begining selection of the restorer lines were carried out according to GSL content. Following selection was aimed at restoring ability in F1 generation after pollination of sterile CMS component. Total 284 restorer lines were tested according their restoring ability. The 65 restorer lines (i.e. 22.89 %) with restoring ability above 90 % were founded. The 45 restorer lines (i.e. 15.84 %) had also GSL content below 18 µmol/g seed. Molecular method using of SCAR marker SG34 was optimallized and successfuly used for markering of Rf restorer gene. Check test evaluation on 50 random selected restorer plants confirmed accuracy of detection dominant Rf gene using these molecular marker. Because of impossibility for recognize dominant homozygous and heterozygous forms of the Rf gene using of fertility restoring test according to fertile flowers in F1 generation in addition to molecular methods Pomocí postupné selekce na nízký obsah glukosinolátů (GSL) a později na schopnost obnovovat fertilitu v F1 generaci po opylení sterilního CMS testera, se podařilo nalézt celkem linie obnovitele fertility s RfRf genem a dobrou kvalitou semene. Celkový počet testovaných linií na schopnost obnovy fertility byl 284. Z tohoto bylo vybráno 65 linií obnovitelů (22,89 %), kteří v F1 generaci restaurovali fertilitu nad 90 %, z toho 45 linií obnovitelů (15,84 %) mělo současně i obsah GSL v semeni pod 18 µmol/g semene. Podařilo se optimalizovat metodu molekulárního markerování Rf genu obnovy fertility s použitím SCAR markeru SG34. Kontrolní hodnocení na 50 náhodně vybraných rostlinách potvrdilo 100 % detekci dominantního Rf genu. Z důvodu problematickém odlišení homozygotního (RfRf) a heterozygotního (Rfrf) alelického páru nesoucího Rf gen, se však nadále uvažuje o využití klasické metody selekce obnovitelů pomocí kontroly fertility v F1 generaci v kombinaci s technikou molekulárního markerování. Po analýze kval
dcterms:title
Utilization of OGU-INRA hybrid system in winter rapeseed breeding. Utilization of OGU-INRA hybrid system in winter rapeseed breeding. VYUŽITÍ HYBRIDNÍHO SYSTÉMU OGU-INRA PŘI ŠLECHTĚNÍ ŘEPKY OZIMÉ
skos:prefLabel
Utilization of OGU-INRA hybrid system in winter rapeseed breeding. VYUŽITÍ HYBRIDNÍHO SYSTÉMU OGU-INRA PŘI ŠLECHTĚNÍ ŘEPKY OZIMÉ Utilization of OGU-INRA hybrid system in winter rapeseed breeding.
skos:notation
RIV/47813644:_____/07:8P009912!RIV08-MZE-47813644
n3:strany
1;12
n3:aktivita
n18:P
n3:aktivity
P(1G46061)
n3:dodaniDat
n4:2008
n3:domaciTvurceVysledku
n17:3473090
n3:druhVysledku
n12:D
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
457153
n3:idVysledku
RIV/47813644:_____/07:8P009912
n3:jazykVysledku
n14:eng
n3:klicovaSlova
winter rapeseed; hybrid; Ogu-INRA; restorer; Rf gene; seed quality; glucosinolates; molecular markers
n3:klicoveSlovo
n5:Rf%20gene n5:glucosinolates n5:hybrid n5:restorer n5:seed%20quality n5:molecular%20markers n5:Ogu-INRA n5:winter%20rapeseed
n3:kontrolniKodProRIV
[3A3B6B844601]
n3:mistoKonaniAkce
MZLU v Brně
n3:mistoVydani
Brno
n3:nazevZdroje
MendelNet 2007
n3:obor
n11:GE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:projekt
n16:1G46061
n3:rokUplatneniVysledku
n4:2007
n3:tvurceVysledku
Macháčková, I. Ehrenbergerová, J. Koprna, Radoslav Horáček, J.
n3:typAkce
n19:WRD
n3:zahajeniAkce
2007-01-01+01:00
s:numberOfPages
12
n13:hasPublisher
Mendelova zemědělská a lesnická univerzita v Brně
n20:isbn
978-80-7375-119-7