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Statements

Subject Item
n2:RIV%2F46747885%3A24410%2F14%3A%230003938%21RIV15-MSM-24410___
rdf:type
skos:Concept n21:Vysledek
rdfs:seeAlso
http://konsys-t.tanger.cz/files/proceedings/20/reports/3481.pdf
dcterms:description
This work is focused on evaluation of scaffolds made by drawing technique for the cell adhesion, viability and proliferation. With drawing we are able to produce very precise fibres with specified orientation from different polymers (PCL, PVA, PVB and others). This characteristic could be an advantage in tissues with ordered pattern. In the primary study the handmade fibres were tested for the cell growth and the results showed the potential of the cells to overgrow the gaps between the fibres. On account of these results further tests were necessary to be done with the precise machine-made fibrous scaffolds. The machine-made fibres are thinner, the gaps between the fibres are smaller and the whole structure is denser. In this study we focused on a planar scaffolds with fibres ordered either in one, in two or in three directions. Mice fibroblasts (3T3 Swiss Albino) were used for the in vitro experiment. The scaffolds were tested at day 1, 3, 7, 14, 21 and 28 after cell seeding. MTT assay, fluorescent microscopy and SEM analysis were used for the evaluation of cell adhesion, viability and proliferation. The results from microscopy analysis as well as from the MTT assay show, that the machine-made scaffolds are capable of supporting cellular attachment and proliferation during our in vitro tests. The scaffolds with crossed fibres show better results compared to fibres ordered in one direction. Nevertheless the one-direction fibres have a great potential in many tissue engineering applications, e.g. for muscles or neural tissue. This work is focused on evaluation of scaffolds made by drawing technique for the cell adhesion, viability and proliferation. With drawing we are able to produce very precise fibres with specified orientation from different polymers (PCL, PVA, PVB and others). This characteristic could be an advantage in tissues with ordered pattern. In the primary study the handmade fibres were tested for the cell growth and the results showed the potential of the cells to overgrow the gaps between the fibres. On account of these results further tests were necessary to be done with the precise machine-made fibrous scaffolds. The machine-made fibres are thinner, the gaps between the fibres are smaller and the whole structure is denser. In this study we focused on a planar scaffolds with fibres ordered either in one, in two or in three directions. Mice fibroblasts (3T3 Swiss Albino) were used for the in vitro experiment. The scaffolds were tested at day 1, 3, 7, 14, 21 and 28 after cell seeding. MTT assay, fluorescent microscopy and SEM analysis were used for the evaluation of cell adhesion, viability and proliferation. The results from microscopy analysis as well as from the MTT assay show, that the machine-made scaffolds are capable of supporting cellular attachment and proliferation during our in vitro tests. The scaffolds with crossed fibres show better results compared to fibres ordered in one direction. Nevertheless the one-direction fibres have a great potential in many tissue engineering applications, e.g. for muscles or neural tissue.
dcterms:title
DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING
skos:prefLabel
DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING DRAWING FIBROUS SCAFFOLDS WITH PRECISE STRUCTURE FOR TISSUE ENGINEERING
skos:notation
RIV/46747885:24410/14:#0003938!RIV15-MSM-24410___
n3:aktivita
n5:P
n3:aktivity
P(ED3.1.00/14.0306)
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n8:3677192 n8:1259474 n8:9851658 n8:3868427 n8:3235939
n3:druhVysledku
n20:D
n3:duvernostUdaju
n9:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
12359
n3:idVysledku
RIV/46747885:24410/14:#0003938
n3:jazykVysledku
n16:eng
n3:klicovaSlova
nanofibres; drawing; scaffolds; tissue engineering
n3:klicoveSlovo
n7:scaffolds n7:drawing n7:nanofibres n7:tissue%20engineering
n3:kontrolniKodProRIV
[2D097E090834]
n3:mistoKonaniAkce
Brno
n3:mistoVydani
Ostrava
n3:nazevZdroje
Nanocon
n3:obor
n11:FP
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n6:ED3.1.00%2F14.0306
n3:rokUplatneniVysledku
n10:2014
n3:tvurceVysledku
Horáková, Jana Mikeš, Petr Jenčová, Věra Pilařová, Kateřina Lukáš, David Stanislav, L.
n3:typAkce
n22:WRD
n3:zahajeniAkce
2014-01-01+01:00
s:numberOfPages
6
n18:hasPublisher
TANGER, Ltd
n13:isbn
978-80-87294-55-0
n15:organizacniJednotka
24410