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Statements

Subject Item

n2:RIV%2F46747885%3A24410%2F13%3A%230001756%21RIV14-GA0-24410___

rdf:type

n6:Vysledek skos:Concept

dcterms:description

Tissue engineering applications widely use nanofibrous scaffolds due to their similarity to native extracellular matrix. Polycaprolactone (PCL) nanofibers have excellent properties for cell attachment. The platelet growth factors promote cell proliferation. Platelet rich plasma is clinically used in chronic wound treatment and in orthopedic applications (nonunionized fractures, total joint replacement). The aim of presented study is the development of nanofibrous layers functionalized by thrombocytes. The effect of platelet growth factors for fibroblast proliferation was tested. PCL nanofibers were immersed for 2 hours in thrombocyte rich solution (TRS) to enable thrombocyte adhesion. These scaffolds were thereafter seeded with fibroblasts. PCL nanofibrous layers without immobilized thrombocytes were used as a control. The presence of thrombocytes after 2 hour incubation was confirmed by scanning electrone microscopy (SEM). Proliferation of seeded fibroblasts on functionalized scaffolds were tested by MTT assay. The nanofibrous scaffold with attached thrombocytes can be used as an efficient drug delivery system for tissue engineering applications. Tissue engineering applications widely use nanofibrous scaffolds due to their similarity to native extracellular matrix. Polycaprolactone (PCL) nanofibers have excellent properties for cell attachment. The platelet growth factors promote cell proliferation. Platelet rich plasma is clinically used in chronic wound treatment and in orthopedic applications (nonunionized fractures, total joint replacement). The aim of presented study is the development of nanofibrous layers functionalized by thrombocytes. The effect of platelet growth factors for fibroblast proliferation was tested. PCL nanofibers were immersed for 2 hours in thrombocyte rich solution (TRS) to enable thrombocyte adhesion. These scaffolds were thereafter seeded with fibroblasts. PCL nanofibrous layers without immobilized thrombocytes were used as a control. The presence of thrombocytes after 2 hour incubation was confirmed by scanning electrone microscopy (SEM). Proliferation of seeded fibroblasts on functionalized scaffolds were tested by MTT assay. The nanofibrous scaffold with attached thrombocytes can be used as an efficient drug delivery system for tissue engineering applications.

dcterms:title

Polycaprolactone Nanofibrous Layer Functionalized by Thrombocyte Rich Solution Polycaprolactone Nanofibrous Layer Functionalized by Thrombocyte Rich Solution

skos:prefLabel

Polycaprolactone Nanofibrous Layer Functionalized by Thrombocyte Rich Solution Polycaprolactone Nanofibrous Layer Functionalized by Thrombocyte Rich Solution

skos:notation

RIV/46747885:24410/13:#0001756!RIV14-GA0-24410___

n6:predkladatel

n18:orjk%3A24410

n4:aktivita

n17:S n17:P

n4:aktivity

P(GAP208/12/0105), S

n4:dodaniDat

n12:2014

n4:domaciTvurceVysledku

n8:1259474 n8:1476548 n8:3677192 n8:9851658

n4:druhVysledku

n7:O

n4:duvernostUdaju

n10:S

n4:entitaPredkladatele

n16:predkladatel

n4:idSjednocenehoVysledku

97058

n4:idVysledku

RIV/46747885:24410/13:#0001756

n4:jazykVysledku

n15:eng

n4:klicovaSlova

Electrospinning, Nanofibers, Polycaprolactone, Thrombocyte Rich Solution

n4:klicoveSlovo

n5:Thrombocyte%20Rich%20Solution n5:Polycaprolactone n5:Electrospinning n5:Nanofibers

n4:kontrolniKodProRIV

[F5DF9559437A]

n4:obor

n14:JJ

n4:pocetDomacichTvurcuVysledku

4

n4:pocetTvurcuVysledku

6

n4:projekt

n11:GAP208%2F12%2F0105

n4:rokUplatneniVysledku

n12:2013

n4:tvurceVysledku

Mikeš, Petr Horáková, Jana Ročková, Kateřina Cudlínová, Marcela Jenčová, Věra Procházková, R.

n13:organizacniJednotka

24410