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Statements

Subject Item
n2:RIV%2F46747885%3A24210%2F12%3A%230003020%21RIV13-GA0-24210___
rdf:type
n7:Vysledek skos:Concept
dcterms:description
The present study was performed to identify possible genotoxicity induced by organic extracts from particulate matter in the exhaust of Cummins ISBe4 and Zetor 1505 diesel engines run on diesel fuel, biodiesel and neat heated fuel-grade rapeseed oil. Diluted exhaust was sampled with high-volume samplers on Teflon coated filters. Filters were extracted with dichlormethane (DCM) and DNA adduct levels induced by extractable organic matter (EOM) in an acellular assay of calf thymus DNA coupled with P-32-postlabeling in the presence and absence of rat liver microsomal S9 fraction were employed. Simultaneously, the chemical analysis of 12 priority PAHs in EOM, including 7 carcinogenic PAHs (c-PAHs) was performed. The results suggest that diesel emissions contain substantially more total PAHs than rapeseed oil emissions (for the ESC) or that these concentrations were comparable (for the WHSC and NRSC), while c-PAHs levels were comparable (for the ESC) or significantly higher (for the WHSC and NRSC) for rapeseed oil emissions. DNA adduct levels induced by diesel and rapeseed oil derived EOM were comparable, but consistently slightly higher for diesel than for rapeseed oil. Highly significant correlations were found between 12 priority PAHs concentrations and DNA adduct levels (0.980; p < 0.001) and these correlations were even stronger for c-PAHs (0.990; p < 0.001). Metabolic activation by the microsomal S9 fraction resulted in several fold higher genotoxicity, suggesting a major contribution of PAHs to genotoxicity. Directly acting compounds, other than c-PAHs, and not requiring S9 to exhibit DNA reactivity were also significant. Generally. DNA adduct levels were more dependent on the type of engine and the test cycle than on the fuel. Our findings suggest that the genotoxicity of particulate emissions from the combustion of rapeseed oil is significant and is comparable to that from the combustion of diesel fuel. The present study was performed to identify possible genotoxicity induced by organic extracts from particulate matter in the exhaust of Cummins ISBe4 and Zetor 1505 diesel engines run on diesel fuel, biodiesel and neat heated fuel-grade rapeseed oil. Diluted exhaust was sampled with high-volume samplers on Teflon coated filters. Filters were extracted with dichlormethane (DCM) and DNA adduct levels induced by extractable organic matter (EOM) in an acellular assay of calf thymus DNA coupled with P-32-postlabeling in the presence and absence of rat liver microsomal S9 fraction were employed. Simultaneously, the chemical analysis of 12 priority PAHs in EOM, including 7 carcinogenic PAHs (c-PAHs) was performed. The results suggest that diesel emissions contain substantially more total PAHs than rapeseed oil emissions (for the ESC) or that these concentrations were comparable (for the WHSC and NRSC), while c-PAHs levels were comparable (for the ESC) or significantly higher (for the WHSC and NRSC) for rapeseed oil emissions. DNA adduct levels induced by diesel and rapeseed oil derived EOM were comparable, but consistently slightly higher for diesel than for rapeseed oil. Highly significant correlations were found between 12 priority PAHs concentrations and DNA adduct levels (0.980; p < 0.001) and these correlations were even stronger for c-PAHs (0.990; p < 0.001). Metabolic activation by the microsomal S9 fraction resulted in several fold higher genotoxicity, suggesting a major contribution of PAHs to genotoxicity. Directly acting compounds, other than c-PAHs, and not requiring S9 to exhibit DNA reactivity were also significant. Generally. DNA adduct levels were more dependent on the type of engine and the test cycle than on the fuel. Our findings suggest that the genotoxicity of particulate emissions from the combustion of rapeseed oil is significant and is comparable to that from the combustion of diesel fuel.
dcterms:title
Genotoxic potential of organic extracts from particle emissions of diesel and rapeseed oil powered engines. Genotoxic potential of organic extracts from particle emissions of diesel and rapeseed oil powered engines.
skos:prefLabel
Genotoxic potential of organic extracts from particle emissions of diesel and rapeseed oil powered engines. Genotoxic potential of organic extracts from particle emissions of diesel and rapeseed oil powered engines.
skos:notation
RIV/46747885:24210/12:#0003020!RIV13-GA0-24210___
n7:predkladatel
n10:orjk%3A24210
n3:aktivita
n14:Z n14:P n14:I
n3:aktivity
I, P(GA101/08/1717), P(GAP503/11/0142), P(GBP503/12/G147), Z(AV0Z50390703)
n3:cisloPeriodika
1
n3:dodaniDat
n17:2013
n3:domaciTvurceVysledku
n15:5758602 n15:3022889 n15:5686997
n3:druhVysledku
n5:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
137984
n3:idVysledku
RIV/46747885:24210/12:#0003020
n3:jazykVysledku
n16:eng
n3:klicovaSlova
diesel engine; toxicity; exhaust; biofuels; biodiesel; rapeseed oil; particulate matter; genotoxicity
n3:klicoveSlovo
n13:biodiesel n13:biofuels n13:toxicity n13:genotoxicity n13:exhaust n13:particulate%20matter n13:diesel%20engine n13:rapeseed%20oil
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[2C9B4BD1D0CE]
n3:nazevZdroje
Toxicology Letters
n3:obor
n18:JT
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:projekt
n4:GA101%2F08%2F1717 n4:GBP503%2F12%2FG147 n4:GAP503%2F11%2F0142
n3:rokUplatneniVysledku
n17:2012
n3:svazekPeriodika
212
n3:tvurceVysledku
Pechout, Martin Schmuczerová, Jana Milcová, Alena Topinka, Jan Vojtíšek, Michal Mazač, Martin
n3:wos
000306887000002
n3:zamer
n8:AV0Z50390703
s:issn
0378-4274
s:numberOfPages
7
n20:doi
10.1016/j.toxlet.2012.04.017
n21:organizacniJednotka
24210