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Statements

Subject Item
n2:RIV%2F26788462%3A_____%2F09%3A%230000216%21RIV10-MSM-26788462
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Incidence of DNA sequences related to toxin genes were studied in 60 isolates of the B. cereus from cow's milk samples using multiplex PCR. All the isolates harboured genes coded for Nhe and EntFM. The hbl genes were detected in 95 % of the isolates. The isolates in our study in the most cases lack the hblA gene. On the other hand all the isolates carried genes nheA, nheB, nheC and entFM. The frequency of the bceT gene was 30.2% in the isolates. Besides, 60.3% of isolates carried the cytK gene (32% cytK1, 28.3% cytK2). Only 1.6 % of the analysed B. cereus isolates was the emetic type. Among all the tested isolates in this study, only one was emetic type, which suggested that emetic type of B. cereus is not predominant in raw cow's milk. Different toxigenic patterns of the analysed isolates indicated that the B. cereus population in cow's milk is diverse. In: Spore forming bacteria in food, Le Berre Media, Quimper, 2009. ISBN 978-2-9514743-2-1. Incidence of DNA sequences related to toxin genes were studied in 60 isolates of the B. cereus from cow's milk samples using multiplex PCR. All the isolates harboured genes coded for Nhe and EntFM. The hbl genes were detected in 95 % of the isolates. The isolates in our study in the most cases lack the hblA gene. On the other hand all the isolates carried genes nheA, nheB, nheC and entFM. The frequency of the bceT gene was 30.2% in the isolates. Besides, 60.3% of isolates carried the cytK gene (32% cytK1, 28.3% cytK2). Only 1.6 % of the analysed B. cereus isolates was the emetic type. Among all the tested isolates in this study, only one was emetic type, which suggested that emetic type of B. cereus is not predominant in raw cow's milk. Different toxigenic patterns of the analysed isolates indicated that the B. cereus population in cow's milk is diverse. In: Spore forming bacteria in food, Le Berre Media, Quimper, 2009. ISBN 978-2-9514743-2-1.
dcterms:title
PCR identification of toxigenic Bacillus cereus isolates from cow´s milk PCR identification of toxigenic Bacillus cereus isolates from cow´s milk
skos:prefLabel
PCR identification of toxigenic Bacillus cereus isolates from cow´s milk PCR identification of toxigenic Bacillus cereus isolates from cow´s milk
skos:notation
RIV/26788462:_____/09:#0000216!RIV10-MSM-26788462
n3:aktivita
n12:P n12:Z
n3:aktivity
P(QF3162), Z(MSM2678846201)
n3:dodaniDat
n19:2010
n3:domaciTvurceVysledku
n14:3470237
n3:druhVysledku
n17:D
n3:duvernostUdaju
n21:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
332910
n3:idVysledku
RIV/26788462:_____/09:#0000216
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Bacillus cereus, toxins, PCR identification, cow milk
n3:klicoveSlovo
n11:cow%20milk n11:PCR%20identification n11:toxins n11:Bacillus%20cereus
n3:kontrolniKodProRIV
[37DEC674B0B0]
n3:mistoKonaniAkce
Quimper
n3:mistoVydani
Quimper
n3:nazevZdroje
Spore forming bacteria in food
n3:obor
n4:EE
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
2
n3:projekt
n20:QF3162
n3:rokUplatneniVysledku
n19:2009
n3:tvurceVysledku
Vyletělová, Marcela Banykó, Juraj
n3:typAkce
n5:EUR
n3:zahajeniAkce
2009-01-01+01:00
n3:zamer
n7:MSM2678846201
s:numberOfPages
3
n18:hasPublisher
Le Berre Media
n16:isbn
978-2-9514743-2-1