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Statements

Subject Item
n2:RIV%2F26784246%3A_____%2F04%3A%230000005%21RIV07-MSM-26784246
rdf:type
n4:Vysledek skos:Concept
dcterms:description
We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop target sequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree. We have used two highly abundant retrotransposons, representative one of the major group of long terminal repeats (LTR) - containing retrotransposons of Ty3/gypsy-like class, Ogre and Cyclop, for development of molecular markers for pea cultivars genotyping. The aim of this study was to access retrotransposon based markers to evaluate the genetic diversity of pea (Pisum sativum L.). To test the utility and reliability of the method we have chosen 20 commercially used pea cultivars with well known pedegree. Due to the sequence knowledge, IRAP markers are better scorable and result in more accurate genetic distance estimations over the REMAP, ISSR and RAPD techniques. With the use of just two outward-facing primers annealing to LTR Ogre and Cyclop target sequences 56 and 43 polymorphic bands per cultivar and reaction were produced which clearly distinguished and separated 20 investigated pea cultivars into groups related to known pedegree. Pomocí dvou vybraných retrotransposonů Ogre a Cyclop byla vyvinuta metoda vhodná pro studium genetické struktury genofondových sbírek harchu. Výhoda této IRAP techniky ve srovnání v jinými je vysoká specificita a zisk dostatečného množství informací postačujících k odlišení i blízce příbuzných odrůd hrachu
dcterms:title
Využitelnost vysoce kopiových retrotransposonových elementů Ogre a cyclop pro molekulární genotypizaci kolekce hrachu. Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm. Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.
skos:prefLabel
Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm. Využitelnost vysoce kopiových retrotransposonových elementů Ogre a cyclop pro molekulární genotypizaci kolekce hrachu. Utility of high copy number Ogre/Cyclop retrotransposons for molecular genotyping of pea (Pisum sativum L.) germplasm.
skos:notation
RIV/26784246:_____/04:#0000005!RIV07-MSM-26784246
n5:strany
326
n5:aktivita
n11:Z
n5:aktivity
Z(MSM2678424601)
n5:dodaniDat
n10:2007
n5:domaciTvurceVysledku
n8:2404257 n8:4071107 n8:7000774 n8:7711700
n5:druhVysledku
n15:D
n5:duvernostUdaju
n20:S
n5:entitaPredkladatele
n14:predkladatel
n5:idSjednocenehoVysledku
591837
n5:idVysledku
RIV/26784246:_____/04:#0000005
n5:jazykVysledku
n13:eng
n5:klicovaSlova
pea, genetic diversity, fingerprinting, retrotransposon
n5:klicoveSlovo
n16:fingerprinting n16:genetic%20diversity n16:pea n16:retrotransposon
n5:kontrolniKodProRIV
[12DCB6513873]
n5:mistoKonaniAkce
Tulln , Rakousko
n5:mistoVydani
Vienna, Rakousko
n5:nazevZdroje
Genetic Variation for Plant Breeding
n5:obor
n18:EB
n5:pocetDomacichTvurcuVysledku
4
n5:pocetTvurcuVysledku
4
n5:rokUplatneniVysledku
n10:2004
n5:tvurceVysledku
Hýbl, Miroslav Smýkal, Petr Griga, Miroslav Dostálová, Radmila
n5:typAkce
n17:EUR
n5:zahajeniAkce
2004-01-01+01:00
n5:zamer
n7:MSM2678424601
s:numberOfPages
1
n19:hasPublisher
BOKU University, Tull
n9:isbn
3-900962-56-1