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Statements

Subject Item
n2:RIV%2F25328859%3A_____%2F10%3A%230000504%21RIV11-MZE-25328859
rdf:type
n7:Vysledek skos:Concept
dcterms:description
Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001 ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases. (C) 2010 Published by Elsevier Ltd. Cochliobolus sativus (anamorph: Bipolaris sorokiniana) is a fungal pathogen that can cause yield limiting diseases of barley and wheat. A PCR-based diagnostic assay was developed to detect B. sorokiniana in barley and wheat tissues using the pathogen-specific primers COSA_F/R derived from the melanin biosynthesis pathway Brn1 locus. Specificity was tested against more than 30 fungal organisms associated with barley and wheat. Limit of detection was determined to be 0.001 ng for pure fungal DNA. The species-specific primers were capable of detecting the pathogen in planta, specifically from infected barley leaves and wheat roots and stem bases. (C) 2010 Published by Elsevier Ltd.
dcterms:title
Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues
skos:prefLabel
Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues Species-specific detection of Bipolaris sorokiniana from wheat and barley tissues
skos:notation
RIV/25328859:_____/10:#0000504!RIV11-MZE-25328859
n3:aktivita
n12:P
n3:aktivity
P(QH71213)
n3:cisloPeriodika
11
n3:dodaniDat
n10:2011
n3:domaciTvurceVysledku
n5:5938759 n5:1503006 n5:5734126 n5:1724541 n5:9024727
n3:druhVysledku
n14:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
289038
n3:idVysledku
RIV/25328859:_____/10:#0000504
n3:jazykVysledku
n17:eng
n3:klicovaSlova
cereal diseases; molecular diagnostics; spot blotch
n3:klicoveSlovo
n9:molecular%20diagnostics n9:spot%20blotch n9:cereal%20diseases
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[72582541C0B9]
n3:nazevZdroje
Crop Protection
n3:obor
n15:GF
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n16:QH71213
n3:rokUplatneniVysledku
n10:2010
n3:svazekPeriodika
29
n3:tvurceVysledku
Spitzer, Tomáš Matušinsky, Pavel Frei, Peter Svačinová, Ivana Mikolášová, Renata Tvarůžek, Ludvík
n3:wos
000282560400015
s:issn
0261-2194
s:numberOfPages
6