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Statements

Subject Item
n2:RIV%2F25271121%3A_____%2F12%3A%230000186%21RIV13-MZE-25271121
rdf:type
skos:Concept n19:Vysledek
rdfs:seeAlso
http://www.nrcresearchpress.com/doi/abs/10.1139/g2012-054
dcterms:description
The aim of this study was to compare traditional hierarchical clustering techniques and principal coordinate analysis (PCoA) with the model-based Bayesian cluster analyses in relation to subpopulation differentiation based on breeding history and geographical origin of apple (Malus x domestica Borkh.) cultivars and landraces. We presented the use of a set of 10 microsatellite (SSR) loci for genetic diversity structure analyses of 273 apple accessions from national genetic resources. These SSR loci yielded a total of 113 polymorphic SSR alleles, with 5-18 alleles per locus. SSR molecular data were successfully used in binary and allelic input format for all genetic diversity analyses, but allelic molecular data did not reveal reliable results with the NTSYS-pc and BAPS softwares. A traditional cluster analysis still provided an easy and effective way for determining genetic diversity structure in the apple germplasm collection. A model-based Bayesian analysis also provided the clustering results in accordance to traditional cluster analysis, but the analyses were distorted by the presence of a dominant group of apple genetic resources owing to the narrow origin of the apple genome. PCoA confirmed that there were no noticeable differences in genetic diversity structure of apple genetic resources during the breeding history. The results of our analyses are useful in the context of enhancing apple collection management, sampling of core collections, and improving breeding processes. The aim of this study was to compare traditional hierarchical clustering techniques and principal coordinate analysis (PCoA) with the model-based Bayesian cluster analyses in relation to subpopulation differentiation based on breeding history and geographical origin of apple (Malus x domestica Borkh.) cultivars and landraces. We presented the use of a set of 10 microsatellite (SSR) loci for genetic diversity structure analyses of 273 apple accessions from national genetic resources. These SSR loci yielded a total of 113 polymorphic SSR alleles, with 5-18 alleles per locus. SSR molecular data were successfully used in binary and allelic input format for all genetic diversity analyses, but allelic molecular data did not reveal reliable results with the NTSYS-pc and BAPS softwares. A traditional cluster analysis still provided an easy and effective way for determining genetic diversity structure in the apple germplasm collection. A model-based Bayesian analysis also provided the clustering results in accordance to traditional cluster analysis, but the analyses were distorted by the presence of a dominant group of apple genetic resources owing to the narrow origin of the apple genome. PCoA confirmed that there were no noticeable differences in genetic diversity structure of apple genetic resources during the breeding history. The results of our analyses are useful in the context of enhancing apple collection management, sampling of core collections, and improving breeding processes.
dcterms:title
Comparison of genetic diversity structure analyses of SSR molecular marker data within apple (Malus x domestica) genetic resources Comparison of genetic diversity structure analyses of SSR molecular marker data within apple (Malus x domestica) genetic resources
skos:prefLabel
Comparison of genetic diversity structure analyses of SSR molecular marker data within apple (Malus x domestica) genetic resources Comparison of genetic diversity structure analyses of SSR molecular marker data within apple (Malus x domestica) genetic resources
skos:notation
RIV/25271121:_____/12:#0000186!RIV13-MZE-25271121
n19:predkladatel
n20:ico%3A25271121
n3:aktivita
n15:P
n3:aktivity
P(QH72163)
n3:cisloPeriodika
9
n3:dodaniDat
n9:2013
n3:domaciTvurceVysledku
n14:6428762 n14:7620330
n3:druhVysledku
n12:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
127933
n3:idVysledku
RIV/25271121:_____/12:#0000186
n3:jazykVysledku
n18:eng
n3:klicovaSlova
BAPS; DARwin; NTSYS-pc; STRUCTURE; principal coordinate analysis
n3:klicoveSlovo
n5:NTSYS-pc n5:BAPS n5:DARwin n5:principal%20coordinate%20analysis n5:STRUCTURE
n3:kodStatuVydavatele
CA - Kanada
n3:kontrolniKodProRIV
[D05D18D1D0E8]
n3:nazevZdroje
GENOME
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
4
n3:projekt
n13:QH72163
n3:rokUplatneniVysledku
n9:2012
n3:svazekPeriodika
55
n3:tvurceVysledku
Patzak, Josef Paprštein, František Henychová, Alena Sedlák, Jiří
n3:wos
000310473300003
s:issn
0831-2796
s:numberOfPages
18
n11:doi
10.1139/G2012-054