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Statements

Subject Item
n2:RIV%2F00843989%3A_____%2F11%3A00102039%21RIV12-MZ0-00843989
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Background: We compared two different agarose isoelectric focusing methods for detection of oligoclonal IgG bands in cerebrospinal fluid and serum: commercial method with immunofixation (Sebia) and home-made method using Multiphor II apparatus followed by affinity immunoblotting. Interobserver agreement for both methods was tested concerning the presence of intrathecal IgG synthesis, the detailed isoelectric focusing pattern type, the number of CSF-restricted oligoclonal IgG bands, and the number of oligoclonal IgG bands in CSF and in serum. Findings: Using kappa statistics for evaluation of agreement, we found there was very good agreement concerning the presence of intrathecal IgG synthesis (kappa 0.870 to 1.000 between methods, and 0.947 and 0.920 between observers, respectively, representing 0 to 5 out of 114 samples classified differently). The agreement was less pronounced when international consensus classification of isoelectric focusing patterns into 5 different types was taken into account (kappa 0.389 to 0.596 between methods); using home-made method, the interobserver agreement regarding pattern type was worse (kappa 0.478) than using commercial Sebia method (kappa 0.791). There was moderate agreement on the number of CSFrestricted oligoclonal IgG bands, and mostly poor agreement on the number of oligoclonal IgG bands in CSF and serum. Conclusions: Both methods were capable to detect oligoclonal IgG reliably, and neither method could be evaluated as superior to the other. However, better interobserver agreement regarding to the pattern type was obtained using commercial Sebia immunofixation method. Rather poor reproducibility of oligoclonal IgG bands numbering should be known to clinicians, since it entails the risk of potentially misleading interpretations. Background: We compared two different agarose isoelectric focusing methods for detection of oligoclonal IgG bands in cerebrospinal fluid and serum: commercial method with immunofixation (Sebia) and home-made method using Multiphor II apparatus followed by affinity immunoblotting. Interobserver agreement for both methods was tested concerning the presence of intrathecal IgG synthesis, the detailed isoelectric focusing pattern type, the number of CSF-restricted oligoclonal IgG bands, and the number of oligoclonal IgG bands in CSF and in serum. Findings: Using kappa statistics for evaluation of agreement, we found there was very good agreement concerning the presence of intrathecal IgG synthesis (kappa 0.870 to 1.000 between methods, and 0.947 and 0.920 between observers, respectively, representing 0 to 5 out of 114 samples classified differently). The agreement was less pronounced when international consensus classification of isoelectric focusing patterns into 5 different types was taken into account (kappa 0.389 to 0.596 between methods); using home-made method, the interobserver agreement regarding pattern type was worse (kappa 0.478) than using commercial Sebia method (kappa 0.791). There was moderate agreement on the number of CSFrestricted oligoclonal IgG bands, and mostly poor agreement on the number of oligoclonal IgG bands in CSF and serum. Conclusions: Both methods were capable to detect oligoclonal IgG reliably, and neither method could be evaluated as superior to the other. However, better interobserver agreement regarding to the pattern type was obtained using commercial Sebia immunofixation method. Rather poor reproducibility of oligoclonal IgG bands numbering should be known to clinicians, since it entails the risk of potentially misleading interpretations.
dcterms:title
Detection of oligoclonal IgG bands in cerebrospinal fluid and serum: comparison between commercial immunofixation method and home-made affinity immunoblotting method and evaluation of interobserver agreement Detection of oligoclonal IgG bands in cerebrospinal fluid and serum: comparison between commercial immunofixation method and home-made affinity immunoblotting method and evaluation of interobserver agreement
skos:prefLabel
Detection of oligoclonal IgG bands in cerebrospinal fluid and serum: comparison between commercial immunofixation method and home-made affinity immunoblotting method and evaluation of interobserver agreement Detection of oligoclonal IgG bands in cerebrospinal fluid and serum: comparison between commercial immunofixation method and home-made affinity immunoblotting method and evaluation of interobserver agreement
skos:notation
RIV/00843989:_____/11:00102039!RIV12-MZ0-00843989
n8:predkladatel
n11:ico%3A00843989
n4:aktivita
n16:V
n4:aktivity
V
n4:cisloPeriodika
4
n4:dodaniDat
n12:2012
n4:domaciTvurceVysledku
n14:1259849 n14:2127741
n4:druhVysledku
n7:J
n4:duvernostUdaju
n17:S
n4:entitaPredkladatele
n13:predkladatel
n4:idSjednocenehoVysledku
193716
n4:idVysledku
RIV/00843989:_____/11:00102039
n4:jazykVysledku
n5:eng
n4:klicovaSlova
oligoclonal IgG bands; different agarose isoelectric focusing methods; cerebrospinal fluid
n4:klicoveSlovo
n9:cerebrospinal%20fluid n9:oligoclonal%20IgG%20bands n9:different%20agarose%20isoelectric%20focusing%20methods
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[1662FA2A0D70]
n4:nazevZdroje
Klinická biochemie a metabolismus
n4:obor
n15:CE
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:rokUplatneniVysledku
n12:2011
n4:svazekPeriodika
19/40
n4:tvurceVysledku
Nováčková, Ludmila Zeman, David
s:issn
1210-7921
s:numberOfPages
5