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Statements

Subject Item
n2:RIV%2F00216305%3A26620%2F14%3APU112580%21RIV15-MSM-26620___
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions. Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions.
dcterms:title
Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza
skos:prefLabel
Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza
skos:notation
RIV/00216305:26620/14:PU112580!RIV15-MSM-26620___
n3:aktivita
n16:O n16:P
n3:aktivity
O, P(ED1.1.00/02.0068)
n3:cisloPeriodika
21-22
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n4:4163435 n4:8184208 n4:2307049 Nguyen, Hoai Viet n4:8541698 n4:4995775
n3:druhVysledku
n17:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
35716
n3:idVysledku
RIV/00216305:26620/14:PU112580
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Protein nucleic acid, Biosensor, Magneticparticle- based separation, Voltammetry
n3:klicoveSlovo
n6:Biosensor n6:Voltammetry n6:Magneticparticle-%20based%20separation n6:Protein%20nucleic%20acid
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[5E65FAA1A0F5]
n3:nazevZdroje
CHROMATOGRAPHIA
n3:obor
n12:CG
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n14:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
77
n3:tvurceVysledku
Guráň, Roman Kizek, René Nguyen, Hoai Viet Krejčová, Ludmila Hynek, David Adam, Vojtěch
s:issn
0009-5893
s:numberOfPages
8
n5:organizacniJednotka
26620