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Statements

Subject Item
n2:RIV%2F00216305%3A26620%2F14%3APU111693%21RIV15-MSM-26620___
rdf:type
n14:Vysledek skos:Concept
dcterms:description
The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. The most important tool in the diagnosis of influenza pandemic is timely and right diagnosis. Hence, this study presents synthesis of paramagnetic particles formed by nanomaghemite (gamma-Fe2O3) core, whose surface was functionalized with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethoxysilane (APTES), able to bind H7N7 influenza virions. Paramagnetic particles show the ability to immobilize virions for direct analysis using ion-exchange chromatography with VIS detection. The presence of H7N7 virions on surface of paramagnetic beads was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry, based upon the mass weight of H7N7 virions, determined before and after establishment of binding. Moreover, using scanning electrochemical microscopy revealed, that binding is provided due to strong charge-charge interaction between The influenza viruses cause annual epidemics of respiratory disease and occasional pandemics, which constitute a major public-health issue. The most important tool in the diagnosis of influenza pandemic is timely and right diagnosis. Hence, this study presents synthesis of paramagnetic particles formed by nanomaghemite (gamma-Fe2O3) core, whose surface was functionalized with tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethoxysilane (APTES), able to bind H7N7 influenza virions. Paramagnetic particles show the ability to immobilize virions for direct analysis using ion-exchange chromatography with VIS detection. The presence of H7N7 virions on surface of paramagnetic beads was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry, based upon the mass weight of H7N7 virions, determined before and after establishment of binding. Moreover, using scanning electrochemical microscopy revealed, that binding is provided due to strong charge-charge interaction between
dcterms:title
gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions
skos:prefLabel
gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions gamma-Fe2O3 Magnetic Core Functionalized with Tetraethyl Orthosilicate and 3-Aminopropyl Triethoxysilane for an Isolation of H7N7 Influenza Serotype Virions
skos:notation
RIV/00216305:26620/14:PU111693!RIV15-MSM-26620___
n3:aktivita
n12:P n12:I
n3:aktivity
I, P(EE2.4.31.0023)
n3:cisloPeriodika
7
n3:dodaniDat
n11:2015
n3:domaciTvurceVysledku
n4:4163435 n4:9584366 n4:8740658 n4:4995775 n4:2307049 n4:6359914 n4:7866283 n4:4733029 Milosavljević, Vedran n4:8947570
n3:druhVysledku
n13:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n8:predkladatel
n3:idSjednocenehoVysledku
17909
n3:idVysledku
RIV/00216305:26620/14:PU111693
n3:jazykVysledku
n9:eng
n3:klicovaSlova
H7N7, Functionalization, Lab-on-a-Chip, Magnetic immobilization, Sensor
n3:klicoveSlovo
n5:Functionalization n5:Lab-on-a-Chip n5:Magnetic%20immobilization n5:Sensor n5:H7N7
n3:kodStatuVydavatele
RS - Srbská republika
n3:kontrolniKodProRIV
[34EF7E48776E]
n3:nazevZdroje
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
n3:obor
n10:CG
n3:pocetDomacichTvurcuVysledku
10
n3:pocetTvurcuVysledku
11
n3:projekt
n18:EE2.4.31.0023
n3:rokUplatneniVysledku
n11:2014
n3:svazekPeriodika
9
n3:tvurceVysledku
Kizek, René Guráň, Roman Heger, Zbyněk Michálek, Petr Cernei, Natalia Vladimirovna Lány, Petr Zítka, Ondřej Milosavljević, Vedran Kynický, Jindřich Kopel, Pavel Adam, Vojtěch
s:issn
1452-3981
s:numberOfPages
12
n17:organizacniJednotka
26620