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Statements

Subject Item
n2:RIV%2F00216305%3A26620%2F14%3APU111671%21RIV15-MSM-26620___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, all these methods require immobilization of biomolecules. Therefore, this is a critical issue in the development of any sort of biosensor affecting its operational properties. In this work, we address this critical issue by employing cyclopropylamine plasma polymerization to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) is attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization are controlled by XPS and FTIR to characterize surface and layer (bulk) chemistry. The results obtained with the antibody linked through the CPA plasma-polymerized layer are comparable with the results obtained previously when the same antibody AL-01 was linked to the surface using th Biosensors have been extensively developed and applied for biomedical and environmental studies. Although there are many different types of biosensing techniques, all these methods require immobilization of biomolecules. Therefore, this is a critical issue in the development of any sort of biosensor affecting its operational properties. In this work, we address this critical issue by employing cyclopropylamine plasma polymerization to deposit stable amine thin films on the surface of quartz crystal microbalance (QCM) biosensors. The antibody specific to human serum albumin (anti-HSA) is attached to the QCM surface via cross-linkage obtained by intermediate reaction with glutaraldehyde. All steps of the bio-immobilization are controlled by XPS and FTIR to characterize surface and layer (bulk) chemistry. The results obtained with the antibody linked through the CPA plasma-polymerized layer are comparable with the results obtained previously when the same antibody AL-01 was linked to the surface using th
dcterms:title
Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application
skos:prefLabel
Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application Cyclopropylamine plasma polymers deposited onto quartz crystal microbalance for biosensing application
skos:notation
RIV/00216305:26620/14:PU111671!RIV15-MSM-26620___
n3:aktivita
n12:P
n3:aktivity
P(ED1.1.00/02.0068)
n3:cisloPeriodika
12
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n5:9040188 n5:5287081
n3:druhVysledku
n15:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n7:predkladatel
n3:idSjednocenehoVysledku
9517
n3:idVysledku
RIV/00216305:26620/14:PU111671
n3:jazykVysledku
n9:eng
n3:klicovaSlova
amine plasma, biosensors, polymers, quartz crystal microbalance, X-ray photoelectron spectroscopy
n3:klicoveSlovo
n10:biosensors n10:amine%20plasma n10:quartz%20crystal%20microbalance n10:X-ray%20photoelectron%20spectroscopy n10:polymers
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[7018609AAD01]
n3:nazevZdroje
physica status solidi (a)
n3:obor
n18:BO
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
7
n3:projekt
n16:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n14:2014
n3:svazekPeriodika
211
n3:tvurceVysledku
Zajíčková, Lenka Čechal, Jan Eliáš, Marek Skládal, Petr Polčák, Josef Manakhov, Anton Nečas, David
s:issn
1862-6300
s:numberOfPages
8
n6:organizacniJednotka
26620