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Statements

Subject Item
n2:RIV%2F00216305%3A26620%2F14%3APU111625%21RIV15-MSM-26620___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
This study was focused on the application of electrochemical methods for studying of bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after theapplication of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 M). Bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like modelorganisms for studying of metals influence to metallothionein expression. Metallothionein was isolatedusing fast protein liquid chromatography and quantified by electrochemical methods. The occurrenceof metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15(MT dimer) and 22 kDa (MT trimer). This study was focused on the application of electrochemical methods for studying of bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after theapplication of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 M). Bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like modelorganisms for studying of metals influence to metallothionein expression. Metallothionein was isolatedusing fast protein liquid chromatography and quantified by electrochemical methods. The occurrenceof metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15(MT dimer) and 22 kDa (MT trimer).
dcterms:title
An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry
skos:prefLabel
An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry
skos:notation
RIV/00216305:26620/14:PU111625!RIV15-MSM-26620___
n3:aktivita
n16:O n16:P
n3:aktivity
O, P(ED1.1.00/02.0068)
n3:cisloPeriodika
SI
n3:dodaniDat
n7:2015
n3:domaciTvurceVysledku
n4:2307049 n4:4163435 Jimenez Jimenez, Ana Maria n4:6044786 n4:1927299 n4:4995775 n4:1774395 n4:2104237 n4:8541698 n4:7957092 n4:1545515
n3:druhVysledku
n17:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
2439
n3:idVysledku
RIV/00216305:26620/14:PU111625
n3:jazykVysledku
n18:eng
n3:klicovaSlova
Escherichia coli, Metallothionein, Voltammetry, Cloning, Metal Ionsa
n3:klicoveSlovo
n9:Escherichia%20coli n9:Cloning n9:Metallothionein n9:Metal%20Ionsa n9:Voltammetry
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[1635596B189D]
n3:nazevZdroje
Electrochimica Acta
n3:obor
n11:CG
n3:pocetDomacichTvurcuVysledku
11
n3:pocetTvurcuVysledku
14
n3:projekt
n14:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n7:2014
n3:svazekPeriodika
140
n3:tvurceVysledku
Číhalová, Kristýna Hynek, David Komínková, Markéta Žůrek, Michal Guráň, Roman Kizek, René Adam, Vojtěch Křížková, Soňa Jimenez Jimenez, Ana Maria Pekařík, Vladimír Tmejová, Kateřina Chudobová, Dagmar Kremplová, Monika Novotná, Marie
n3:wos
000342528600003
s:issn
0013-4686
s:numberOfPages
9
n15:organizacniJednotka
26620