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Statements

Subject Item
n2:RIV%2F00216305%3A26620%2F14%3APU110753%21RIV15-MSM-26620___
rdf:type
n17:Vysledek skos:Concept
dcterms:description
Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 L. The flow rate was optimized to 500 L/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticlesmodified with glucose. The detection limit of the whole assay, which included capturing of ba Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 L. The flow rate was optimized to 500 L/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticlesmodified with glucose. The detection limit of the whole assay, which included capturing of ba
dcterms:title
Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment
skos:prefLabel
Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment
skos:notation
RIV/00216305:26620/14:PU110753!RIV15-MSM-26620___
n3:aktivita
n19:O n19:P
n3:aktivity
O, P(ED1.1.00/02.0068)
n3:cisloPeriodika
14
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n6:7413645 n6:6359914 n6:5530237 n6:5314801 n6:1545515 n6:2307049 n6:8541698 n6:4995775 n6:5238439 n6:3649857 n6:7026048 n6:9211705 n6:1927299 n6:9821643 n6:2104237 n6:4797760
n3:druhVysledku
n16:J
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
42151
n3:idVysledku
RIV/00216305:26620/14:PU110753
n3:jazykVysledku
n7:eng
n3:klicovaSlova
Alkaline phosphatase, Bacteria, Electrochemical detection, Magnetic particles, 1-Naphthyl phosphate, Planetary science, Remote sensing
n3:klicoveSlovo
n4:Alkaline%20phosphatase n4:1-Naphthyl%20phosphate n4:Planetary%20science n4:Electrochemical%20detection n4:Remote%20sensing n4:Magnetic%20particles n4:Bacteria
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[4A33073F3EE1]
n3:nazevZdroje
Electrophoresis
n3:obor
n12:CG
n3:pocetDomacichTvurcuVysledku
16
n3:pocetTvurcuVysledku
16
n3:projekt
n18:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n14:2014
n3:svazekPeriodika
35
n3:tvurceVysledku
Žůrek, Michal Kizek, René Chudobová, Dagmar Kudr, Jiří Prášek, Jan Burian, František Ruttkay-Nedecký, Branislav Adam, Vojtěch Kopečný, Lukáš Novotná, Marie Hynek, David Číhalová, Kristýna Nejdl, Lukáš Žalud, Luděk Křížková, Soňa Kopel, Pavel
n3:wos
000340394800009
s:issn
0173-0835
s:numberOfPages
13
n13:doi
10.1002/elps.201300576
n5:organizacniJednotka
26620