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Statements

Subject Item
n2:RIV%2F00216305%3A26620%2F14%3APU108609%21RIV15-MSM-26620___
rdf:type
n10:Vysledek skos:Concept
dcterms:description
We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored usingUV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragmentof DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted intoa metal bound DNA (Zn–DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum(190–350 nm) and decrease in the melting temperature (Tm) of Zn–DNA. Spectrophotometric (UV/vis)analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at200 nm and decrease in absorbance at 251 nm. We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored usingUV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragmentof DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted intoa metal bound DNA (Zn–DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum(190–350 nm) and decrease in the melting temperature (Tm) of Zn–DNA. Spectrophotometric (UV/vis)analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at200 nm and decrease in absorbance at 251 nm.
dcterms:title
DNA interaction with zinc(II) ions DNA interaction with zinc(II) ions
skos:prefLabel
DNA interaction with zinc(II) ions DNA interaction with zinc(II) ions
skos:notation
RIV/00216305:26620/14:PU108609!RIV15-MSM-26620___
n3:aktivita
n14:P
n3:aktivity
P(EE2.4.31.0023), P(GAP102/11/1068)
n3:cisloPeriodika
3
n3:dodaniDat
n16:2015
n3:domaciTvurceVysledku
n4:6794181 n4:6359914 n4:3790185 n4:2104237 n4:2032279 n4:2645572 n4:5314801 n4:2307049 n4:9600426 n4:4995775
n3:druhVysledku
n9:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n18:predkladatel
n3:idSjednocenehoVysledku
11997
n3:idVysledku
RIV/00216305:26620/14:PU108609
n3:jazykVysledku
n17:eng
n3:klicovaSlova
UV/vis spectrophotometry, Gel electrophoresis, Melting temperature
n3:klicoveSlovo
n7:UV%2Fvis%20spectrophotometry n7:Gel%20electrophoresis n7:Melting%20temperature
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[7CC974A2942C]
n3:nazevZdroje
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
n3:obor
n12:CG
n3:pocetDomacichTvurcuVysledku
10
n3:pocetTvurcuVysledku
13
n3:projekt
n11:EE2.4.31.0023 n11:GAP102%2F11%2F1068
n3:rokUplatneniVysledku
n16:2014
n3:svazekPeriodika
64
n3:tvurceVysledku
Křížková, Soňa Kizek, René Dostálová, Simona Nejdl, Lukáš Kudr, Jiří Babula, Petr Šmerková, Kristýna Kopel, Pavel Zehnálek, Josef Vaculovičová, Markéta Ruttkay-Nedecký, Branislav Trnková, Libuše Adam, Vojtěch
s:issn
0141-8130
s:numberOfPages
7
n8:organizacniJednotka
26620