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Statements

Subject Item
n2:RIV%2F00216305%3A26620%2F13%3APU104787%21RIV14-MSM-26620___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
In this study, we determined serum levels of metallothioneins (MTs) and zinc in children with solid tumours (neuroblastoma, Hodgkin lymphoma, medulloblastoma, osteosarcoma, Ewing sarcoma and nephroblastoma) by differential pulse voltammetry Brdicka reaction and ELISA. Zn(II) level in patients sera was 40% compared to controls, contrariwise, MT level was 4.2 higher in patients. No significant differences among single diagnoses were found both for Zn(II) and MT. When determined Zn(II)/MT ratio, in controls its value was 24.6, but it was 2.6 in patients. After Western-blotting with anti-MT and anti-Zn chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for MT- and Zn blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimised algorithm. In this study, we determined serum levels of metallothioneins (MTs) and zinc in children with solid tumours (neuroblastoma, Hodgkin lymphoma, medulloblastoma, osteosarcoma, Ewing sarcoma and nephroblastoma) by differential pulse voltammetry Brdicka reaction and ELISA. Zn(II) level in patients sera was 40% compared to controls, contrariwise, MT level was 4.2 higher in patients. No significant differences among single diagnoses were found both for Zn(II) and MT. When determined Zn(II)/MT ratio, in controls its value was 24.6, but it was 2.6 in patients. After Western-blotting with anti-MT and anti-Zn chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for MT- and Zn blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimised algorithm.
dcterms:title
Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours
skos:prefLabel
Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours
skos:notation
RIV/00216305:26620/13:PU104787!RIV14-MSM-26620___
n11:predkladatel
n12:orjk%3A26620
n3:aktivita
n19:I n19:O n19:P
n3:aktivity
I, O, P(ED1.1.00/02.0068)
n3:cisloPeriodika
11
n3:dodaniDat
n13:2014
n3:domaciTvurceVysledku
n10:2307049 n10:4995775 n10:2104237 n10:8541698
n3:druhVysledku
n18:J
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
112947
n3:idVysledku
RIV/00216305:26620/13:PU104787
n3:jazykVysledku
n4:eng
n3:klicovaSlova
wavelet transformation, metallothionein, Western blot
n3:klicoveSlovo
n5:Western%20blot n5:wavelet%20transformation n5:metallothionein
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[BF49F3C3CFC7]
n3:nazevZdroje
Electrophoresis
n3:obor
n15:FD
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
10
n3:projekt
n6:ED1.1.00%2F02.0068
n3:rokUplatneniVysledku
n13:2013
n3:svazekPeriodika
34
n3:tvurceVysledku
Anýz, Jiří Hynek, David Kizek, René Křížková, Soňa Kruseová, Jarmila Hraběta, Jan Štěpánková, Olga Eckschlager, Tomáš Vysloužilová, Lenka Adam, Vojtěch
s:issn
0173-0835
s:numberOfPages
12
n14:organizacniJednotka
26620