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Statements

Subject Item
n2:RIV%2F00216305%3A26310%2F14%3APU109810%21RIV15-GA0-26310___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Isolation of DNA is an important step in the molecular diagnostics of microorganisms and viruses, medical diagnosis, etc. Different magnetic nano- and microparticles have been used as a solid support for DNA isolation. Magnetic nano- and microparticles usually consist of a number of components: supermagnetic core, protective coating and the surface functionality. Iron oxides and some substances used to stabilize magnetic cores reduce biocompatibility of the proposed carriers, therefore perfectly cover core prerequisite for their application in molecular diagnostics. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). The work was focused on the estimation of influence of microparticles with magnetite core on the amplification of DNA. A set of 8 types of hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) – P(HEMA-co-GMA) microparticles, 6 types of poly(glycidyl methacrylate) (PGMA) microparticles and 3 types of Isolation of DNA is an important step in the molecular diagnostics of microorganisms and viruses, medical diagnosis, etc. Different magnetic nano- and microparticles have been used as a solid support for DNA isolation. Magnetic nano- and microparticles usually consist of a number of components: supermagnetic core, protective coating and the surface functionality. Iron oxides and some substances used to stabilize magnetic cores reduce biocompatibility of the proposed carriers, therefore perfectly cover core prerequisite for their application in molecular diagnostics. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). The work was focused on the estimation of influence of microparticles with magnetite core on the amplification of DNA. A set of 8 types of hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) – P(HEMA-co-GMA) microparticles, 6 types of poly(glycidyl methacrylate) (PGMA) microparticles and 3 types of
dcterms:title
The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR
skos:prefLabel
The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR
skos:notation
RIV/00216305:26310/14:PU109810!RIV15-GA0-26310___
n3:aktivita
n16:P
n3:aktivity
P(GP13-32840P)
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n14:3954633 n14:4977459 n14:2412594
n3:druhVysledku
n17:O
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
48295
n3:idVysledku
RIV/00216305:26310/14:PU109810
n3:jazykVysledku
n5:eng
n3:klicovaSlova
magnetic particles, PCR inhibition
n3:klicoveSlovo
n8:PCR%20inhibition n8:magnetic%20particles
n3:kontrolniKodProRIV
[B1F5629BF229]
n3:obor
n13:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n11:GP13-32840P
n3:rokUplatneniVysledku
n10:2014
n3:tvurceVysledku
Trachtová, Štěpánka Horák, Daniel Rittich, Bohuslav Španová, Alena
n15:organizacniJednotka
26310