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Statements

Subject Item
n2:RIV%2F00216305%3A26310%2F14%3APU108364%21RIV15-MSM-26310___
rdf:type
n15:Vysledek skos:Concept
dcterms:description
Dynamic light scattering (DLS) was used to monitor the coil–globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6,000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6,000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6,000 concentration ranging from 5.5 to 8 %. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16 % PEG 6,000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of mic Dynamic light scattering (DLS) was used to monitor the coil–globule transition of calf thymus and bacterial DNAs by poly(ethylene glycol) (PEG 600 and PEG 6,000) in aqueous NaCl solutions. The contribution of PEG 600 and PEG 6,000 to DNA coil diameter change was investigated. Compaction of DNA molecules was observed for a PEG 6,000 concentration ranging from 5.5 to 8 %. Hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA), poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), and poly(glycidyl methacrylate) (PGMA) microspheres containing carboxyl groups were used for DNA isolation. The highest DNA yield was achieved using 16 % PEG 6,000 and 2.0 M NaCl. The amount of isolated DNA correlated with the content of carboxyl groups on the microsphere surface. RNA adsorption on the surface of the microspheres tested was also studied. RNA recovery was more than one order lower than DNA recovery under the same conditions. All types of mic
dcterms:title
Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres
skos:prefLabel
Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres Solid-phase DNA isolation from food matrices using hydrophilic magnetic microspheres
skos:notation
RIV/00216305:26310/14:PU108364!RIV15-MSM-26310___
n3:aktivita
n19:S n19:P
n3:aktivity
P(GP13-32840P), S
n3:cisloPeriodika
3
n3:dodaniDat
n5:2015
n3:domaciTvurceVysledku
n10:3954633 n10:4977459 n10:2412594 Prettl, Zsolt
n3:druhVysledku
n16:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
45892
n3:idVysledku
RIV/00216305:26310/14:PU108364
n3:jazykVysledku
n14:eng
n3:klicovaSlova
DNA compaction, Magnetic microspheres, DNA isolation, RNA, Polymerase chain reaction (PCR)
n3:klicoveSlovo
n4:DNA%20isolation n4:DNA%20compaction n4:RNA n4:Magnetic%20microspheres n4:Polymerase%20chain%20reaction%20%28PCR%29
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[804A6345AA82]
n3:nazevZdroje
FOOD AND BIOPRODUCTS PROCESSING
n3:obor
n17:EB
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
7
n3:projekt
n13:GP13-32840P
n3:rokUplatneniVysledku
n5:2014
n3:svazekPeriodika
92
n3:tvurceVysledku
Gyenis, János Horák, Daniel Prettl, Zsolt Španová, Alena Tóth, Judit Rittich, Bohuslav Trachtová, Štěpánka
s:issn
0960-3085
s:numberOfPages
26
n8:doi
10.1016/j.fbp.2014.05.001
n18:organizacniJednotka
26310