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Statements

Subject Item
n2:RIV%2F00216305%3A26310%2F11%3APU94248%21RIV12-MSM-26310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
The aim of this study was to analyze the set of 43 Clostridium strains for volatile carboxylic acids production. Species-specific PCRs for Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. Formation of short-chain carboxylic acids (acetic, propionic, 2-methylpropanoic, butyric and valeric) was studied by gas chromatography in culture supernatants. For each of them, Kovats index was estimated. Most of the strains had produced acetic acid (40 strains) and butyric acid (33 strains), while 2-methylpropanoic acid was determined in 14 samples, propionic acid in 11 samples and valeric acid had been produced only by 7 strains. The highest concentrations were measured for acetic acid and butyric acid. Total concentration of analyzed acids was in the range from 0.4 to 6 mg/ml. The chromatographic profiles of produced carboxylic acids were in no correlation with the species identification (Clostridium butyricum and Clostridium The aim of this study was to analyze the set of 43 Clostridium strains for volatile carboxylic acids production. Species-specific PCRs for Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. Formation of short-chain carboxylic acids (acetic, propionic, 2-methylpropanoic, butyric and valeric) was studied by gas chromatography in culture supernatants. For each of them, Kovats index was estimated. Most of the strains had produced acetic acid (40 strains) and butyric acid (33 strains), while 2-methylpropanoic acid was determined in 14 samples, propionic acid in 11 samples and valeric acid had been produced only by 7 strains. The highest concentrations were measured for acetic acid and butyric acid. Total concentration of analyzed acids was in the range from 0.4 to 6 mg/ml. The chromatographic profiles of produced carboxylic acids were in no correlation with the species identification (Clostridium butyricum and Clostridium
dcterms:title
Production of carboxylic acids by different strains of Clostridium Production of carboxylic acids by different strains of Clostridium
skos:prefLabel
Production of carboxylic acids by different strains of Clostridium Production of carboxylic acids by different strains of Clostridium
skos:notation
RIV/00216305:26310/11:PU94248!RIV12-MSM-26310___
n11:predkladatel
n12:orjk%3A26310
n3:aktivita
n21:S n21:P
n3:aktivity
P(2B08070), S
n3:dodaniDat
n19:2012
n3:domaciTvurceVysledku
n5:4977459 n5:2893789 n5:2412594 n5:3875091 Gregušová, Barbora
n3:druhVysledku
n20:D
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n22:predkladatel
n3:idSjednocenehoVysledku
224263
n3:idVysledku
RIV/00216305:26310/11:PU94248
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Clostridium sp., gas chromatography, volatile carboxylic acids
n3:klicoveSlovo
n13:Clostridium%20sp. n13:volatile%20carboxylic%20acids n13:gas%20chromatography
n3:kontrolniKodProRIV
[C4248B0FD62D]
n3:mistoKonaniAkce
Častá-Papiernička
n3:mistoVydani
Trnava
n3:nazevZdroje
International Conference Applied Natural Sciences 2011 Papiernička - Častá, October 5 - 7 PROCEEDINGS
n3:obor
n18:EI
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n9:2B08070
n3:rokUplatneniVysledku
n19:2011
n3:tvurceVysledku
Rittich, Bohuslav Španová, Alena Dubský, Hanniel Gregušová, Barbora Dvořák, Miloš
n3:typAkce
n16:WRD
n3:zahajeniAkce
2011-10-05+02:00
s:numberOfPages
5
n4:hasPublisher
University of SS. Cyril and Methodius in Trnava
n7:isbn
978-80-8105-266-8
n8:organizacniJednotka
26310