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Statements

Subject Item
n2:RIV%2F00216305%3A26310%2F10%3APU93667%21RIV12-MSM-26310___
rdf:type
skos:Concept n14:Vysledek
dcterms:description
Molecular biological methods (genus-specific PCR, species-specific PCRs, RAPD with primer M13, and rep-PCR with primer (GTG)5) were applied for the identification and discrimination of 31 strains of the Lactobacillus genus isolated from faecal samples of 30 breastfed infants. All strains were tested for bile salts and low pH tolerance, growth in milk, production of lactic acids in milk supplemented with yeast extract, and used in biochemical tests including production of beta-glucuronidase. Consequently, the presence of genes coding for decarboxylases was investigated using PCR. Finally, four strains of probiotic species were selected for use in the dairy industry and for other tests concerning their probiotic character. Molecular biological methods (genus-specific PCR, species-specific PCRs, RAPD with primer M13, and rep-PCR with primer (GTG)5) were applied for the identification and discrimination of 31 strains of the Lactobacillus genus isolated from faecal samples of 30 breastfed infants. All strains were tested for bile salts and low pH tolerance, growth in milk, production of lactic acids in milk supplemented with yeast extract, and used in biochemical tests including production of beta-glucuronidase. Consequently, the presence of genes coding for decarboxylases was investigated using PCR. Finally, four strains of probiotic species were selected for use in the dairy industry and for other tests concerning their probiotic character.
dcterms:title
Characterisation of potential probiotic Lactobacillus strains of human origin Characterisation of potential probiotic Lactobacillus strains of human origin
skos:prefLabel
Characterisation of potential probiotic Lactobacillus strains of human origin Characterisation of potential probiotic Lactobacillus strains of human origin
skos:notation
RIV/00216305:26310/10:PU93667!RIV12-MSM-26310___
n3:aktivita
n12:S
n3:aktivity
S
n3:dodaniDat
n7:2012
n3:domaciTvurceVysledku
n10:7340958 n10:2412594 n10:4977459
n3:druhVysledku
n16:O
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n13:predkladatel
n3:idSjednocenehoVysledku
250389
n3:idVysledku
RIV/00216305:26310/10:PU93667
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Lactobacillus, identification, PCR, RAPD, rep-PCR, probiotics, in vitro tests
n3:klicoveSlovo
n6:identification n6:probiotics n6:PCR n6:RAPD n6:Lactobacillus n6:in%20vitro%20tests n6:rep-PCR
n3:kontrolniKodProRIV
[DCBCC21A4C03]
n3:obor
n11:EB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
6
n3:rokUplatneniVysledku
n7:2010
n3:tvurceVysledku
Španová, Alena Turková, Kristýna Rittich, Bohuslav
n9:organizacniJednotka
26310