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Statements

Subject Item
n2:RIV%2F00216305%3A26220%2F11%3APU94013%21RIV15-MSM-26220___
rdf:type
n9:Vysledek skos:Concept
dcterms:description
Low-molecular mass proteins rich in cysteines called metallothioneins (MT) can be considered as markers for the pollution of the environment by metals. Here, we report on suggestion for an automated procedure for the isolation of MT followed by voltammetric analysis. Primarily, we optimized the automated detection of MTusing an electrochemical analyser. It was found that the most sensitive and repeatable analyses are obtained at a temperature of 4C for the supporting electrolyte. Further, we optimized experimental conditions for the isolation of MT by using antibody-linked paramagnetic microparticles. Under the optimal conditions (4 h long interaction between the microparticles and MT), the microparticles were tested on isolation of various amounts of MT. The lowest isolated amount of MT by antibody-linked paramagnetic microparticles was 5 mg ml of MT (50 ng). The automated procedure of MT isolation was further tested on isolation of MT from guppy fish (Poecilia reticulata) treated with silver(I) ions Low-molecular mass proteins rich in cysteines called metallothioneins (MT) can be considered as markers for the pollution of the environment by metals. Here, we report on suggestion for an automated procedure for the isolation of MT followed by voltammetric analysis. Primarily, we optimized the automated detection of MTusing an electrochemical analyser. It was found that the most sensitive and repeatable analyses are obtained at a temperature of 4C for the supporting electrolyte. Further, we optimized experimental conditions for the isolation of MT by using antibody-linked paramagnetic microparticles. Under the optimal conditions (4 h long interaction between the microparticles and MT), the microparticles were tested on isolation of various amounts of MT. The lowest isolated amount of MT by antibody-linked paramagnetic microparticles was 5 mg ml of MT (50 ng). The automated procedure of MT isolation was further tested on isolation of MT from guppy fish (Poecilia reticulata) treated with silver(I) ions
dcterms:title
Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution
skos:prefLabel
Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution Paramagnetic antibody-modified microparticles coupled with voltammetry as a tool for isolation and detection of metallothionen as a bioindicator of metal pollution
skos:notation
RIV/00216305:26220/11:PU94013!RIV15-MSM-26220___
n3:aktivita
n14:Z n14:S n14:P
n3:aktivity
P(GA102/08/1546), P(KAN208130801), S, Z(MSM0021622412), Z(MSM0021630513), Z(MSM6215712402)
n3:cisloPeriodika
13
n3:dodaniDat
n15:2015
n3:domaciTvurceVysledku
n4:4489705 n4:2307049 n4:4995775 n4:8088586 n4:7210655
n3:druhVysledku
n6:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
219525
n3:idVysledku
RIV/00216305:26220/11:PU94013
n3:jazykVysledku
n16:eng
n3:klicovaSlova
antibody, microparticles, voltammetry, metallothionen, pollution
n3:klicoveSlovo
n7:microparticles n7:antibody n7:pollution n7:metallothionen n7:voltammetry
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[963303298918]
n3:nazevZdroje
JOURNAL OF ENVIRONMENTAL MONITORING
n3:obor
n10:CE
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
9
n3:projekt
n19:KAN208130801 n19:GA102%2F08%2F1546
n3:rokUplatneniVysledku
n15:2011
n3:svazekPeriodika
2011
n3:tvurceVysledku
Hubálek, Jaromír Provazník, Ivo Trnková, Libuše Kizek, René Adam, Vojtěch Beklová, Miroslava Škutková, Helena Húska, Dalibor
n3:zamer
n11:MSM0021622412 n11:MSM6215712402 n11:MSM0021630513
s:issn
1464-0325
s:numberOfPages
7
n8:organizacniJednotka
26220