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Statements

Subject Item
n2:RIV%2F00216305%3A26220%2F09%3APU85269%21RIV10-AV0-26220___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrod This paper reports on the analysis of specific sequence of Phage Lambda DNA amplified by PCR. Agarose gel electrophoresis, gel electrophoresis on chip and stationary electrochemical instrument were employed for detection of amplicons obtained after 2, 4, 6, 8, 10, 15, 20, 25, 30 and 35 cycles. In the case of agarose gel electrophoresis the lowest detectable amount of DNA was obtained after 15 PCR cycles. Gel electrophoresis on chip offers higher sensitivity because the lowest detectable amount of amplicons by this technique was obtained after eight PCR cycles. Further we employed square wave voltammetry and various working electrodes (hanging mercury drop electrode, screen-printed carbon electrode and carbon-nanotube-based screen-printed electrodes) to detect amplicons. Amplicons obtained even after two cycles were detectable at all electrodes. To improve the selectivity of electrochemical detection carbon nanoelectrodes were off-line coupled with gel electrophoresis. Into the agarose gel the electrod
dcterms:title
Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR
skos:prefLabel
Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR Miniaturized electrochemical detector as a tool for detection of DNA amplified by PCR
skos:notation
RIV/00216305:26220/09:PU85269!RIV10-AV0-26220___
n3:aktivita
n11:P
n3:aktivity
P(KAN208130801)
n3:cisloPeriodika
24
n3:dodaniDat
n15:2010
n3:domaciTvurceVysledku
n13:4489705
n3:druhVysledku
n12:J
n3:duvernostUdaju
n7:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
326413
n3:idVysledku
RIV/00216305:26220/09:PU85269
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Carbon nanotubes, DNA, Gel electrophoresis on chip, PCR, Voltammetry
n3:klicoveSlovo
n6:PCR n6:DNA n6:Carbon%20nanotubes n6:Voltammetry n6:Gel%20electrophoresis%20on%20chip
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[5E99A23BB1EB]
n3:nazevZdroje
Electrophoresis
n3:obor
n14:CG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
4
n3:projekt
n9:KAN208130801
n3:rokUplatneniVysledku
n15:2009
n3:svazekPeriodika
2008 (29)
n3:tvurceVysledku
Kizek, René Adam, Vojtěch Hubálek, Jaromír Húska, Dalibor
s:issn
0173-0835
s:numberOfPages
8
n18:organizacniJednotka
26220