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Statements

Subject Item
n2:RIV%2F00216305%3A26210%2F12%3APU99555%21RIV13-MSM-26210___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Computerised x-ray microtomography (microCT) is increasingly used in the 3D study of bone microarchitecture and in quantifying bone volume fractions. However, the volumetric resolution in labora- tory apparatus for small rodent studies is at best several microns linear and recognisable detail characterising forming, resting and resorbing surfaces is completely missing. Backscattered electron mode scanning electron microscopy (BSE SEM) of both macerated 3D samples and polished surfaces of blocks of PMMA embedded tissue provides this information but samples have to be cut and processed. The same PMMA material is good for confocal fluores- cence microscopy (CSLM) for both tissue morphology and the study of tetracycline and calcein mineralising front labels to 50– 200 microns deep to the block surface. With the recent acquisi- tion of SEM with variable chamber pressure to permit examina- tion of uncoated specimens, we are able to conduct CSLM after SEM for correlation studies. Here, we report new approa Computerised x-ray microtomography (microCT) is increasingly used in the 3D study of bone microarchitecture and in quantifying bone volume fractions. However, the volumetric resolution in labora- tory apparatus for small rodent studies is at best several microns linear and recognisable detail characterising forming, resting and resorbing surfaces is completely missing. Backscattered electron mode scanning electron microscopy (BSE SEM) of both macerated 3D samples and polished surfaces of blocks of PMMA embedded tissue provides this information but samples have to be cut and processed. The same PMMA material is good for confocal fluores- cence microscopy (CSLM) for both tissue morphology and the study of tetracycline and calcein mineralising front labels to 50– 200 microns deep to the block surface. With the recent acquisi- tion of SEM with variable chamber pressure to permit examina- tion of uncoated specimens, we are able to conduct CSLM after SEM for correlation studies. Here, we report new approa
dcterms:title
Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM
skos:prefLabel
Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM Correlation between 3D imaging methods in studying bone architecture: SEM, microCT and confocal LM
skos:notation
RIV/00216305:26210/12:PU99555!RIV13-MSM-26210___
n16:predkladatel
n17:orjk%3A26210
n3:aktivita
n4:I
n3:aktivity
I
n3:dodaniDat
n6:2013
n3:domaciTvurceVysledku
n7:2173069 n7:9676090
n3:druhVysledku
n9:O
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
128856
n3:idVysledku
RIV/00216305:26210/12:PU99555
n3:jazykVysledku
n12:eng
n3:klicovaSlova
x-ray microtomography,scanning electron microscopy,confocal fluorescence microscopy,rat femur
n3:klicoveSlovo
n10:scanning%20electron%20microscopy n10:confocal%20fluorescence%20microscopy n10:rat%20femur n10:x-ray%20microtomography
n3:kontrolniKodProRIV
[ED72F99AC9C0]
n3:obor
n5:BO
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n6:2012
n3:tvurceVysledku
Boyde, Alan Zikmund, Tomáš Kvasnica, Lukáš
n14:organizacniJednotka
26210