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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F14%3A39899343%21RIV15-MSM-25310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
Nowadays, supercritical fluid chromatography (SFC) has a renaissance in analytical chemistry due to the commercial availability of reliable SFC systems with potential applications for fast separations of compounds with low to medium polarity, such as lipids. SFC / mass spectrometry (SFC/MS) coupling brings some advantages over conventional (U)HPLC/MS systems, especially in terms of reduced analysis time and therefore higher throughput in the lipidomic analysis. The basic approach for the lipidomic quantitation is based on the direct infusion (shotgun) without any chromatography, but this technique could suffer from suppression effects and the lack of structural details, such as the differentiation of isobaric species. (U)HPLC/MS can be used either for lipid class or lipid species separation. Lipid class separation is achieved by hydrophilic-liquid chromatography (HILIC) for polar lipids or normal-phase (NP) HPLC for nonpolar lipids. Reversed-phase (RP) HPLC enables the resolution of lipid species differing in alkyl chain lengths and the number and positions of double bonds. The combination of HILIC and RP is two-dimensional separation in offline or online modes provides the most detailed characterization due to a good orthogonality of both separation modes. SFC can be successfully applied for the class separation of both polar and nonpolar lipid classes in one run. At least 16 lipid classes together with 2 internal standards can be separated and quantified within 6 minutes. The coupling with high-resolution Q-TOF mass analyzer equipped with ion mobility separation brings another dimension of separation and structural confirmation for complex lipidomic studies, which is applied for the analysis of tumor samples of patients with selected types of cancer. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic). Nowadays, supercritical fluid chromatography (SFC) has a renaissance in analytical chemistry due to the commercial availability of reliable SFC systems with potential applications for fast separations of compounds with low to medium polarity, such as lipids. SFC / mass spectrometry (SFC/MS) coupling brings some advantages over conventional (U)HPLC/MS systems, especially in terms of reduced analysis time and therefore higher throughput in the lipidomic analysis. The basic approach for the lipidomic quantitation is based on the direct infusion (shotgun) without any chromatography, but this technique could suffer from suppression effects and the lack of structural details, such as the differentiation of isobaric species. (U)HPLC/MS can be used either for lipid class or lipid species separation. Lipid class separation is achieved by hydrophilic-liquid chromatography (HILIC) for polar lipids or normal-phase (NP) HPLC for nonpolar lipids. Reversed-phase (RP) HPLC enables the resolution of lipid species differing in alkyl chain lengths and the number and positions of double bonds. The combination of HILIC and RP is two-dimensional separation in offline or online modes provides the most detailed characterization due to a good orthogonality of both separation modes. SFC can be successfully applied for the class separation of both polar and nonpolar lipid classes in one run. At least 16 lipid classes together with 2 internal standards can be separated and quantified within 6 minutes. The coupling with high-resolution Q-TOF mass analyzer equipped with ion mobility separation brings another dimension of separation and structural confirmation for complex lipidomic studies, which is applied for the analysis of tumor samples of patients with selected types of cancer. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic).
dcterms:title
Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples
skos:prefLabel
Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples Comparison of SFC/MS and UHPLC/MS techniques in the lipidomic characterization of biological samples
skos:notation
RIV/00216275:25310/14:39899343!RIV15-MSM-25310___
n4:aktivita
n5:P
n4:aktivity
P(LL1302)
n4:dodaniDat
n13:2015
n4:domaciTvurceVysledku
n9:8944164 n9:1030787 n9:4329082
n4:druhVysledku
n8:O
n4:duvernostUdaju
n15:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
8194
n4:idVysledku
RIV/00216275:25310/14:39899343
n4:jazykVysledku
n10:eng
n4:klicovaSlova
lipidomic characterization; UHPLC/MS; SFC/MS
n4:klicoveSlovo
n6:SFC%2FMS n6:UHPLC%2FMS n6:lipidomic%20characterization
n4:kontrolniKodProRIV
[35509367DE15]
n4:obor
n7:CB
n4:pocetDomacichTvurcuVysledku
3
n4:pocetTvurcuVysledku
3
n4:projekt
n17:LL1302
n4:rokUplatneniVysledku
n13:2014
n4:tvurceVysledku
Cífková, Eva Lísa, Miroslav Holčapek, Michal
n11:organizacniJednotka
25310