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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F14%3A39899340%21RIV15-MSM-25310___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Lipidomics deals with the complex lipidomic characterization of dynamic biological systems. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but on the other hand the advantage is that the sample is infused at the constant eluent composition and flow rate, so the suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is another techniques, which is often used without a chromatographic separation, but the well-known limitation of MALDI for the quantitative purposes is less signal stability and reproducibility depending on the matrix used and the sample preparation. The systematic optimization of matrix selection and deposition approaches (spraying, electrospraying and sublimation) or matrix-free techniques on dedicated metal plates shows a potential of MALDI/LDI for the (semi-)quantitative lipidomic analysis. We will present examples of individual separation modes suitable for distinguishing of particular type of isomerism, such as reversed-phase, silver-ion or chiral chromatography used either in conventional HPLC mode or preferably under UHPLC conditions using sub-2 mým particles and high back pressures or alternatively core-shell sub-3 mým particles. Recently, the potential of supercritical fluid chromatography (SFC) has been realized with a great separation efficiency and very short analysis times compared to LC/MS. Various quantitative LC/MS and SFC/MS approaches will be discussed as well. Ion mobility used in configuration with mass spectrometry can bring supplementary separation of lipids from background ions and separation of lipid classes and species inside classes. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic). Lipidomics deals with the complex lipidomic characterization of dynamic biological systems. The ion suppression effects could reduce the sensitivity for determination of trace lipids, but on the other hand the advantage is that the sample is infused at the constant eluent composition and flow rate, so the suppression effects can be compensated by internal standards and the quantitative data are reliable, as demonstrated in numerous large-scale clinical studies. Matrix-assisted laser desorption/ionization (MALDI)-MS is another techniques, which is often used without a chromatographic separation, but the well-known limitation of MALDI for the quantitative purposes is less signal stability and reproducibility depending on the matrix used and the sample preparation. The systematic optimization of matrix selection and deposition approaches (spraying, electrospraying and sublimation) or matrix-free techniques on dedicated metal plates shows a potential of MALDI/LDI for the (semi-)quantitative lipidomic analysis. We will present examples of individual separation modes suitable for distinguishing of particular type of isomerism, such as reversed-phase, silver-ion or chiral chromatography used either in conventional HPLC mode or preferably under UHPLC conditions using sub-2 mým particles and high back pressures or alternatively core-shell sub-3 mým particles. Recently, the potential of supercritical fluid chromatography (SFC) has been realized with a great separation efficiency and very short analysis times compared to LC/MS. Various quantitative LC/MS and SFC/MS approaches will be discussed as well. Ion mobility used in configuration with mass spectrometry can bring supplementary separation of lipids from background ions and separation of lipid classes and species inside classes. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic).
dcterms:title
Comparison of Mass Spectrometry Based Approaches in Lipidomics Comparison of Mass Spectrometry Based Approaches in Lipidomics
skos:prefLabel
Comparison of Mass Spectrometry Based Approaches in Lipidomics Comparison of Mass Spectrometry Based Approaches in Lipidomics
skos:notation
RIV/00216275:25310/14:39899340!RIV15-MSM-25310___
n3:aktivita
n11:P
n3:aktivity
P(LL1302)
n3:dodaniDat
n7:2015
n3:domaciTvurceVysledku
Chagovets, Vitaliy Viktorovich n9:1030787 n9:4329082 n9:8944164 n9:6345883
n3:druhVysledku
n14:O
n3:duvernostUdaju
n17:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
8117
n3:idVysledku
RIV/00216275:25310/14:39899340
n3:jazykVysledku
n15:eng
n3:klicovaSlova
Lipidomics; Mass Spectrometry
n3:klicoveSlovo
n12:Mass%20Spectrometry n12:Lipidomics
n3:kontrolniKodProRIV
[EE0ECCDB5FB0]
n3:obor
n13:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n10:LL1302
n3:rokUplatneniVysledku
n7:2014
n3:tvurceVysledku
Cífková, Eva Lísa, Miroslav Chagovets, Vitaliy Viktorovich Holčapek, Michal Jirásko, Robert
n16:organizacniJednotka
25310