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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F14%3A39899334%21RIV15-MSM-25310___
rdf:type
skos:Concept n17:Vysledek
dcterms:description
Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the fast separation of 16 nonpolar and polar lipid classes within 6 minute analysis including the partial separation of some species inside individual classes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification of individual species. The composition of esterified fatty acids and polar head groups are characterized based on their characteristic fragment ions obtained from data independent analysis with high and low collision energy. IM is used as an additional separation dimension enabling the separation of lipid classes together with the separation of individual lipid species differing in acyl chain lengths and the number of double bonds coeluting in SFC. Developed SFC/IM-MS method is applied for the comprehensive analysis of lipid composition in real lipid samples. Obtained data from analyzed samples are processed using Progenesis QI software followed by the statistical analysis. Fast SFC analysis is developed for the separation of nonpolar and polar lipid classes in one analysis followed by IM-MS identification of individual species. Developed SFC/IM-MS approach enables high-throughput nontargeted lipidomic analysis of complex lipid samples. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic). Individual parameters of SFC analysis have been carefully optimized to achieve a maximum number of separated lipid classes. Developed SFC method enables the fast separation of 16 nonpolar and polar lipid classes within 6 minute analysis including the partial separation of some species inside individual classes. Mass spectra with high mass accuracy and resolving power are acquired using ESI in both positive- and negative-ion modes for the identification of individual species. The composition of esterified fatty acids and polar head groups are characterized based on their characteristic fragment ions obtained from data independent analysis with high and low collision energy. IM is used as an additional separation dimension enabling the separation of lipid classes together with the separation of individual lipid species differing in acyl chain lengths and the number of double bonds coeluting in SFC. Developed SFC/IM-MS method is applied for the comprehensive analysis of lipid composition in real lipid samples. Obtained data from analyzed samples are processed using Progenesis QI software followed by the statistical analysis. Fast SFC analysis is developed for the separation of nonpolar and polar lipid classes in one analysis followed by IM-MS identification of individual species. Developed SFC/IM-MS approach enables high-throughput nontargeted lipidomic analysis of complex lipid samples. This work was supported by ERC CZ project No. LL1302 (MSMT, Czech Republic).
dcterms:title
Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids
skos:prefLabel
Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids Lipidomics: Supercritical fluid chromatography/ion mobility - mass spectrometry as a tool for fast nontargeted analysis of lipids
skos:notation
RIV/00216275:25310/14:39899334!RIV15-MSM-25310___
n3:aktivita
n16:P
n3:aktivity
P(LL1302)
n3:dodaniDat
n10:2015
n3:domaciTvurceVysledku
n7:4329082 n7:1030787
n3:druhVysledku
n14:O
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
26312
n3:idVysledku
RIV/00216275:25310/14:39899334
n3:jazykVysledku
n13:eng
n3:klicovaSlova
lipids; mass spectrometry; ion mobility; Supercritical fluid chromatography; Lipidomics
n3:klicoveSlovo
n4:lipids n4:Supercritical%20fluid%20chromatography n4:mass%20spectrometry n4:Lipidomics n4:ion%20mobility
n3:kontrolniKodProRIV
[852AA4B2C204]
n3:obor
n5:CB
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
2
n3:projekt
n15:LL1302
n3:rokUplatneniVysledku
n10:2014
n3:tvurceVysledku
Lísa, Miroslav Holčapek, Michal
n9:organizacniJednotka
25310