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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F14%3A39898252%21RIV15-MSM-25310___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance liquid chromatography method for the determination of retinol and alpha-tocopherol in human seminal plasma was developed. Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance liquid chromatography method for the determination of retinol and alpha-tocopherol in human seminal plasma was developed.
dcterms:title
Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection
skos:prefLabel
Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection
skos:notation
RIV/00216275:25310/14:39898252!RIV15-MSM-25310___
n3:aktivita
n8:I
n3:aktivity
I
n3:cisloPeriodika
5
n3:dodaniDat
n13:2015
n3:domaciTvurceVysledku
n9:2726955 n9:8771928 n9:1997475
n3:druhVysledku
n7:J
n3:duvernostUdaju
n16:S
n3:entitaPredkladatele
n4:predkladatel
n3:idSjednocenehoVysledku
10812
n3:idVysledku
RIV/00216275:25310/14:39898252
n3:jazykVysledku
n18:eng
n3:klicovaSlova
tocopherol; seminal plasma; retinol; high-performance liquid chromatography; Butylated hydroxytoluene
n3:klicoveSlovo
n12:retinol n12:high-performance%20liquid%20chromatography n12:Butylated%20hydroxytoluene n12:seminal%20plasma n12:tocopherol
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[C7AA106F1BFA]
n3:nazevZdroje
Andrologia
n3:obor
n17:CE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:rokUplatneniVysledku
n13:2014
n3:svazekPeriodika
46
n3:tvurceVysledku
Myslíková, Kateřina Hampl, Radek Kanďár, Roman Drábková, Petra
n3:wos
000335009600003
s:issn
0303-4569
s:numberOfPages
7
n11:doi
10.1111/and.12103
n15:organizacniJednotka
25310