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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F14%3A39898080%21RIV15-MSM-25310___
rdf:type
n11:Vysledek skos:Concept
dcterms:description
Tau protein plays a crucial role in the neuronal cytoskeleton stabilization. Under the pathological conditions, it can be abnormally phosphorylated which leads to the aggregation and formation of neurofibrillary tangles representing pathological hallmark of Alzheimer's disease (AD). For its association with neurodegenerative diseases, tau protein is intensively studied in various diagnostic and therapeutic applications. Since there is no standard of tau protein involving essential post-translational modifications, it is often necessary to purify it directly from cerebrospinal fluid (CSF) or blood of healthy or AD clinical signs exhibiting organism. The immunomagnetic purification based on the isolation of the target protein using a specific antibody bound to a magnetic carrier is the most effective tool for this purpose. High quality antibodies are the main prerequisite of successful purification, but many commercial antibodies do not comply with the challenging requirements for the immunosorbent preparation. In this work, we compared four different anti-tau monoclonal antibodies currently available on the market (clones HT7, BT2, 8F10, 7E5). The evaluation criteria were set along the intended use for the preparation of specific magnetic immunosorbent subsequently applicable for the native tau protein purification. We evaluated the characteristics declared by producers as specificity, purity and homogeneity. We also tested the binding affinity and IgG stability during the covalent immobilization to the surface of magnetic microparticles and during the immunoprecipitation of intact tau protein or tryptic tau fragments. The results are summarized and discussed here. Tau protein plays a crucial role in the neuronal cytoskeleton stabilization. Under the pathological conditions, it can be abnormally phosphorylated which leads to the aggregation and formation of neurofibrillary tangles representing pathological hallmark of Alzheimer's disease (AD). For its association with neurodegenerative diseases, tau protein is intensively studied in various diagnostic and therapeutic applications. Since there is no standard of tau protein involving essential post-translational modifications, it is often necessary to purify it directly from cerebrospinal fluid (CSF) or blood of healthy or AD clinical signs exhibiting organism. The immunomagnetic purification based on the isolation of the target protein using a specific antibody bound to a magnetic carrier is the most effective tool for this purpose. High quality antibodies are the main prerequisite of successful purification, but many commercial antibodies do not comply with the challenging requirements for the immunosorbent preparation. In this work, we compared four different anti-tau monoclonal antibodies currently available on the market (clones HT7, BT2, 8F10, 7E5). The evaluation criteria were set along the intended use for the preparation of specific magnetic immunosorbent subsequently applicable for the native tau protein purification. We evaluated the characteristics declared by producers as specificity, purity and homogeneity. We also tested the binding affinity and IgG stability during the covalent immobilization to the surface of magnetic microparticles and during the immunoprecipitation of intact tau protein or tryptic tau fragments. The results are summarized and discussed here.
dcterms:title
QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN
skos:prefLabel
QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN QUALITY EVALUATION OF MONOCLONAL ANTIBODIES SUITABLE FOR IMMUNOMAGNETIC PURIFICATION OF NATIVE TAU PROTEIN
skos:notation
RIV/00216275:25310/14:39898080!RIV15-MSM-25310___
n4:aktivita
n12:P
n4:aktivity
P(7E12083), P(EE2.3.30.0021)
n4:cisloPeriodika
září 2014
n4:dodaniDat
n16:2015
n4:domaciTvurceVysledku
n5:9213627 n5:2568756 n5:2189429 n5:9624376
n4:druhVysledku
n15:J
n4:duvernostUdaju
n8:S
n4:entitaPredkladatele
n6:predkladatel
n4:idSjednocenehoVysledku
41023
n4:idVysledku
RIV/00216275:25310/14:39898080
n4:jazykVysledku
n17:eng
n4:klicovaSlova
monoclonal antibody; immunomagnetic purification; Alzheimer's disease; Tau protein
n4:klicoveSlovo
n9:Tau%20protein n9:immunomagnetic%20purification n9:monoclonal%20antibody n9:Alzheimer%27s%20disease
n4:kodStatuVydavatele
CZ - Česká republika
n4:kontrolniKodProRIV
[B3B9BC57C1AB]
n4:nazevZdroje
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
n4:obor
n14:EC
n4:pocetDomacichTvurcuVysledku
4
n4:pocetTvurcuVysledku
6
n4:projekt
n13:EE2.3.30.0021 n13:7E12083
n4:rokUplatneniVysledku
n16:2014
n4:svazekPeriodika
20
n4:tvurceVysledku
Kašparová, Jitka Jankovičová, Barbora Hromádková, Lenka Kupčík, Rudolf Řípová, Daniela Bílková, Zuzana
s:issn
1211-5541
s:numberOfPages
17
n10:organizacniJednotka
25310