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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F14%3A39897974%21RIV15-MSM-25310___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Hyaluronic acid (HA) is known to serve as a dynamic mediator intervening in many physiological functions. Its specific effect has been repeatedly confirmed to be strongly influenced by the molecular size of hyaluronan fragments. However common technological approaches of HA fragments production have their limitations. In many cases, the final products do not meet the strict pharmaceutical requirements, specifically due to size polydispersity and reaction contaminants. We present novel methodology based on combination of unique incidental ability of the plant-derived protease papain to split the glycosidic bonds and an indispensable advantages of biocompatible macroporous material with incorporated ferrous ions serving as carrier for covalent papain fixation. This atypical and yet unpublished highly efficient multiparametric approach allows enhanced HA fragmentation for easily and safely producing molar-mass-defined HA fragments with narrow size distribution. Native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography/multi-angle light scattering (SEC-MALS) confirmed the effectiveness of our multiparametric approach. Hyaluronic acid (HA) is known to serve as a dynamic mediator intervening in many physiological functions. Its specific effect has been repeatedly confirmed to be strongly influenced by the molecular size of hyaluronan fragments. However common technological approaches of HA fragments production have their limitations. In many cases, the final products do not meet the strict pharmaceutical requirements, specifically due to size polydispersity and reaction contaminants. We present novel methodology based on combination of unique incidental ability of the plant-derived protease papain to split the glycosidic bonds and an indispensable advantages of biocompatible macroporous material with incorporated ferrous ions serving as carrier for covalent papain fixation. This atypical and yet unpublished highly efficient multiparametric approach allows enhanced HA fragmentation for easily and safely producing molar-mass-defined HA fragments with narrow size distribution. Native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography/multi-angle light scattering (SEC-MALS) confirmed the effectiveness of our multiparametric approach.
dcterms:title
Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments
skos:prefLabel
Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments Enhanced multiparametric hyaluronan degradation for production of molar-mass-defined fragments
skos:notation
RIV/00216275:25310/14:39897974!RIV15-MSM-25310___
n3:aktivita
n8:P
n3:aktivity
P(EE2.3.30.0021)
n3:cisloPeriodika
November
n3:dodaniDat
n14:2015
n3:domaciTvurceVysledku
n5:5486076 n5:6708714 n5:3796876 n5:9213627 n5:8213992
n3:druhVysledku
n9:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n16:predkladatel
n3:idSjednocenehoVysledku
14673
n3:idVysledku
RIV/00216275:25310/14:39897974
n3:jazykVysledku
n12:eng
n3:klicovaSlova
Pharmaceutics; SEC-MALS; Magnetic particles; Papain; Hyaluronan fragmentation
n3:klicoveSlovo
n4:Hyaluronan%20fragmentation n4:Pharmaceutics n4:Papain n4:SEC-MALS n4:Magnetic%20particles
n3:kodStatuVydavatele
GB - Spojené království Velké Británie a Severního Irska
n3:kontrolniKodProRIV
[7AD0AE6496F3]
n3:nazevZdroje
Carbohydrate Polymers
n3:obor
n18:FR
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
8
n3:projekt
n10:EE2.3.30.0021
n3:rokUplatneniVysledku
n14:2014
n3:svazekPeriodika
112
n3:tvurceVysledku
Ehlová, Tereza Bílková, Zuzana Podzimek, Štěpán Rotková, Jana Korecká, Lucie Velebný, Vladimír Moravcová, Veronika Holubová, Lucie
n3:wos
000341464600037
s:issn
0144-8617
s:numberOfPages
6
n17:doi
10.1016/j.carbpol.2014.05.096
n7:organizacniJednotka
25310