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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F13%3A39897015%21RIV14-MSM-25310___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
The selection of antibodies with the desired specificity, immunoreactivity, purity and integrity is a key step in developing an effective immunosorbent for immunomagnetic separation (IMS). The choice of methods for characterizing the antibodies is limited, especially with antibodies specific to surface cell structures. In this study, four commercial anti-Salmonella antibodies were evaluated from the point of view of their immunoreactivity with the cells of Salmonella Typhimurium and of their purity. For these purposes, traditional SDS-PAGE analysis with subsequent silver staining and a newly adapted whole-cell dot blot technique were applied. Based on this testing, monoclonal anti-core LPS Salmonella antibodies from MyBiosource unambiguously demonstrated the highest immunoreactivity to Salmonella Typhimurium cells, whereas cross-reactivity with the closely related G- bacteria, Escherichia coli and Citrobacter braakii was not observed. Such antibodies will be subsequently used for the preparation of an anti-Salmonella immunosorbent and applied in IMS. The immunoreactivity and selectivity of anti-Salmonella poly- and monoclonal antibodies are also discussed. The selection of antibodies with the desired specificity, immunoreactivity, purity and integrity is a key step in developing an effective immunosorbent for immunomagnetic separation (IMS). The choice of methods for characterizing the antibodies is limited, especially with antibodies specific to surface cell structures. In this study, four commercial anti-Salmonella antibodies were evaluated from the point of view of their immunoreactivity with the cells of Salmonella Typhimurium and of their purity. For these purposes, traditional SDS-PAGE analysis with subsequent silver staining and a newly adapted whole-cell dot blot technique were applied. Based on this testing, monoclonal anti-core LPS Salmonella antibodies from MyBiosource unambiguously demonstrated the highest immunoreactivity to Salmonella Typhimurium cells, whereas cross-reactivity with the closely related G- bacteria, Escherichia coli and Citrobacter braakii was not observed. Such antibodies will be subsequently used for the preparation of an anti-Salmonella immunosorbent and applied in IMS. The immunoreactivity and selectivity of anti-Salmonella poly- and monoclonal antibodies are also discussed.
dcterms:title
Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot
skos:prefLabel
Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot Quality evaluation of commercial anti-Salmonella antibodies for immunomagnetic separation using whole-cell dot blot
skos:notation
RIV/00216275:25310/13:39897015!RIV14-MSM-25310___
n10:predkladatel
n11:orjk%3A25310
n3:aktivita
n14:P
n3:aktivity
P(7E13029), P(EE2.3.30.0021)
n3:cisloPeriodika
prosinec
n3:dodaniDat
n18:2014
n3:domaciTvurceVysledku
n4:1646834 n4:9213627 n4:5486076 n4:2568756 n4:7886950 n4:3491625
n3:druhVysledku
n12:J
n3:duvernostUdaju
n5:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
101154
n3:idVysledku
RIV/00216275:25310/13:39897015
n3:jazykVysledku
n17:eng
n3:klicovaSlova
quality evaluation; whole-cell dot-blot; anti-Salmonella antibodies; imunosorbent; imunomagnetic separation of bacteria
n3:klicoveSlovo
n9:quality%20evaluation n9:anti-Salmonella%20antibodies n9:imunosorbent n9:imunomagnetic%20separation%20of%20bacteria n9:whole-cell%20dot-blot
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[CC6B38F37F25]
n3:nazevZdroje
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
n3:obor
n7:CB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n16:7E13029 n16:EE2.3.30.0021
n3:rokUplatneniVysledku
n18:2013
n3:svazekPeriodika
19
n3:tvurceVysledku
Bílková, Zuzana Holubová, Lucie Dvořáková, Veronika Kučerová, Jana Jankovičová, Barbora Moťková, Petra
s:issn
1211-5541
s:numberOfPages
10
n8:organizacniJednotka
25310