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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F13%3A39896743%21RIV14-GA0-25310___
rdf:type
n12:Vysledek skos:Concept
dcterms:description
Selecting the right monoclonal antibody (mAb) for an immunoaffinity-based application can be tricky, as many mAb producers offer a wide range of mAb clones against molecular structures of interest. Since there are significant differences in the quality of mAb clones, and particularly in their binding activity, an easy method for quick and low-cost comparison of various mAb clones was developed. The dot-ELISA affinity test is a simple, versatile and instrumentally no demanding technique, since it requires no expensive equipment (such as an ELISA reader or chemiluminescence/fluorescence imaging system) and can be performed in any biochemical laboratory. This method is based on a previously described dot-ELISA technique that is improved with a chaotropic step using different concentrations of ammonium thiocyanate in the range 0-2 M. In this work, the dot-ELISA affinity test was optimized on Abeta peptide as antigen and anti-Abeta mAb. Such protocol was then applied to a panel of eight anti-EpCAM (epithelial cell adhesion molecule) mAbs which should be subsequently used for preparation of magnetic immunosorbent to capture circulating tumor cells (CTCs). Selecting the right monoclonal antibody (mAb) for an immunoaffinity-based application can be tricky, as many mAb producers offer a wide range of mAb clones against molecular structures of interest. Since there are significant differences in the quality of mAb clones, and particularly in their binding activity, an easy method for quick and low-cost comparison of various mAb clones was developed. The dot-ELISA affinity test is a simple, versatile and instrumentally no demanding technique, since it requires no expensive equipment (such as an ELISA reader or chemiluminescence/fluorescence imaging system) and can be performed in any biochemical laboratory. This method is based on a previously described dot-ELISA technique that is improved with a chaotropic step using different concentrations of ammonium thiocyanate in the range 0-2 M. In this work, the dot-ELISA affinity test was optimized on Abeta peptide as antigen and anti-Abeta mAb. Such protocol was then applied to a panel of eight anti-EpCAM (epithelial cell adhesion molecule) mAbs which should be subsequently used for preparation of magnetic immunosorbent to capture circulating tumor cells (CTCs).
dcterms:title
Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies
skos:prefLabel
Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies
skos:notation
RIV/00216275:25310/13:39896743!RIV14-GA0-25310___
n12:predkladatel
n13:orjk%3A25310
n3:aktivita
n8:P n8:I
n3:aktivity
I, P(7E09080), P(7E09109), P(7E12053), P(7E12083), P(EE2.3.30.0021), P(GAP206/12/0381)
n3:cisloPeriodika
3
n3:dodaniDat
n5:2014
n3:domaciTvurceVysledku
n7:2568756 n7:4136950 n7:9213627
n3:druhVysledku
n20:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
70495
n3:idVysledku
RIV/00216275:25310/13:39896743
n3:jazykVysledku
n11:eng
n3:klicovaSlova
Abeta peptides; EpCAM; Chaotropic elution; Monoclonal antibody; Affinity; Dot-ELISA; Dot blot
n3:klicoveSlovo
n4:Chaotropic%20elution n4:Affinity n4:Monoclonal%20antibody n4:Abeta%20peptides n4:Dot%20blot n4:Dot-ELISA n4:EpCAM
n3:kodStatuVydavatele
RO - Rumunsko
n3:kontrolniKodProRIV
[2465348E4A98]
n3:nazevZdroje
Journal of Analytical and Bioanalytical Techniques
n3:obor
n18:EC
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n10:7E09080 n10:7E12053 n10:7E12083 n10:7E09109 n10:EE2.3.30.0021 n10:GAP206%2F12%2F0381
n3:rokUplatneniVysledku
n5:2013
n3:svazekPeriodika
4
n3:tvurceVysledku
Horák, Daniel Jankovičová, Barbora Bílková, Zuzana Svobodová, Zuzana
s:issn
2155-9872
s:numberOfPages
5
n6:doi
10.4172/2155-9872.1000168
n16:organizacniJednotka
25310