This HTML5 document contains 48 embedded RDF statements represented using HTML+Microdata notation.

The embedded RDF content will be recognized by any processor of HTML5 Microdata.

Namespace Prefixes

PrefixIRI
dctermshttp://purl.org/dc/terms/
n3http://localhost/temp/predkladatel/
n16http://linked.opendata.cz/resource/domain/vavai/riv/tvurce/
n12http://linked.opendata.cz/resource/domain/vavai/vysledek/RIV%2F00216275%3A25310%2F13%3A39896383%21RIV14-MSM-25310___/
n9http://linked.opendata.cz/resource/domain/vavai/subjekt/
n8http://linked.opendata.cz/ontology/domain/vavai/
shttp://schema.org/
skoshttp://www.w3.org/2004/02/skos/core#
rdfshttp://www.w3.org/2000/01/rdf-schema#
n4http://linked.opendata.cz/ontology/domain/vavai/riv/
n2http://linked.opendata.cz/resource/domain/vavai/vysledek/
rdfhttp://www.w3.org/1999/02/22-rdf-syntax-ns#
n5http://linked.opendata.cz/ontology/domain/vavai/riv/klicoveSlovo/
n19http://linked.opendata.cz/ontology/domain/vavai/riv/duvernostUdaju/
xsdhhttp://www.w3.org/2001/XMLSchema#
n18http://linked.opendata.cz/ontology/domain/vavai/riv/aktivita/
n14http://linked.opendata.cz/ontology/domain/vavai/riv/jazykVysledku/
n17http://linked.opendata.cz/ontology/domain/vavai/riv/druhVysledku/
n10http://linked.opendata.cz/ontology/domain/vavai/riv/obor/
n7http://reference.data.gov.uk/id/gregorian-year/

Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F13%3A39896383%21RIV14-MSM-25310___
rdf:type
n8:Vysledek skos:Concept
rdfs:seeAlso
http://www.electrochemsci.org/papers/vol8/80709030.pdf
dcterms:description
An amperometric biosensor for the determination of hydroquinone and other polyphenolic antioxidants is presented, based on a carbon paste electrode on which tyrosinase is immobilized in a Nafion (R) film. In a 0.1 M phosphate buffer (pH 7.0 and 25 +/- 1 degrees C), the antioxidant capacity can be expressed as the concentration equivalent of p-hydroquinone (HQ), according to an amperometric response of polyphenolic antioxidants with the carbon paste sensor at -0.24 V vs. Ag/AgCl/3 M KCl. In the range of low HQ concentrations (up to 120 mu mol L-1), the corresponding calibration curve follows the equation I [mu A] = -0.0426 -0.0113 c(HQ) [mu mol L-1] with a correlation coefficient R = 0.999. The limit of detection (LOD for S/N = 3) was estimated as 1.6 mu mol L-1, the repeatability was 1.2 % RSD (c = 90 mu M, n = 5 measurements). The biosensor can be applied to determine the phenolic antioxidant capacity in plants and food samples in the absence of higher concentrations of ascorbic acid. An amperometric biosensor for the determination of hydroquinone and other polyphenolic antioxidants is presented, based on a carbon paste electrode on which tyrosinase is immobilized in a Nafion (R) film. In a 0.1 M phosphate buffer (pH 7.0 and 25 +/- 1 degrees C), the antioxidant capacity can be expressed as the concentration equivalent of p-hydroquinone (HQ), according to an amperometric response of polyphenolic antioxidants with the carbon paste sensor at -0.24 V vs. Ag/AgCl/3 M KCl. In the range of low HQ concentrations (up to 120 mu mol L-1), the corresponding calibration curve follows the equation I [mu A] = -0.0426 -0.0113 c(HQ) [mu mol L-1] with a correlation coefficient R = 0.999. The limit of detection (LOD for S/N = 3) was estimated as 1.6 mu mol L-1, the repeatability was 1.2 % RSD (c = 90 mu M, n = 5 measurements). The biosensor can be applied to determine the phenolic antioxidant capacity in plants and food samples in the absence of higher concentrations of ascorbic acid.
dcterms:title
Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity
skos:prefLabel
Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity Amperometric Enzyme Carbon Paste-Based Biosensor for Quantification of Hydroquinone and Polyphenolic Antioxidant Capacity
skos:notation
RIV/00216275:25310/13:39896383!RIV14-MSM-25310___
n8:predkladatel
n9:orjk%3A25310
n4:aktivita
n18:I n18:S
n4:aktivity
I, S
n4:cisloPeriodika
7
n4:dodaniDat
n7:2014
n4:domaciTvurceVysledku
n16:8488568 n16:6143083
n4:druhVysledku
n17:J
n4:duvernostUdaju
n19:S
n4:entitaPredkladatele
n12:predkladatel
n4:idSjednocenehoVysledku
60254
n4:idVysledku
RIV/00216275:25310/13:39896383
n4:jazykVysledku
n14:eng
n4:klicovaSlova
red wine analysis; hydroquinone; antioxidant capacity; tyrosinase; biosensor; amperometry; carbon paste electrode
n4:klicoveSlovo
n5:antioxidant%20capacity n5:tyrosinase n5:biosensor n5:carbon%20paste%20electrode n5:hydroquinone n5:amperometry n5:red%20wine%20analysis
n4:kodStatuVydavatele
CS - Státní svaz Srbska a Černé Hory
n4:kontrolniKodProRIV
[553F074B537E]
n4:nazevZdroje
International Journal of Electrochemical Science
n4:obor
n10:CG
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
4
n4:rokUplatneniVysledku
n7:2013
n4:svazekPeriodika
8
n4:tvurceVysledku
Kalcher, Kurt Sýs, Milan Pekec, Bruna Vytřas, Karel
n4:wos
000323547600010
s:issn
1452-3981
s:numberOfPages
11
n3:organizacniJednotka
25310