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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F13%3A39896312%21RIV14-GA0-25310___
rdf:type
n14:Vysledek skos:Concept
rdfs:seeAlso
http://www.sciencedirect.com/science/article/pii/S0021967313000228
dcterms:description
Due to ever increasing importance of short analysis times in modern HPLC practice, fast gradient elution is becoming widely used both in one-dimensional and two-dimensional separations, especially in the second dimension where the speed of separation is of primary importance. For method development and optimization, prediction of retention in gradient elution from the isocratic data is very important. This is principally possible using the well-established theory of gradient elution, especially for reversed-phase separations. Feasibility of these approaches has been proven in conventional liquid chromatography and commercial prediction software is available for this purpose. However, fast gradient chromatography employing short columns and gradient times in between 1 and 2 min or even less, impose additional stringent demands on the accuracy of prediction, due to rapid changes in mobile phase composition and increased role of extra-column contributions and instrumental dwell volume on the retention. In the present work, possibilities of prediction of the gradient elution times from the isocratic data measured only at two mobile phase concentrations were investigated in fast chromatography of alkylbenzenes, phenylurea and triazine pesticides on five columns differing in lengths: two monolithic ones and three packed with particles of different size. In the second dimension, gradient times are strongly restricted by the short cycle frequency. The effects of the flow-rate on the elution times, on the accuracy of their prediction and on peak capacity were studied under time constraint conditions. Gradient theory can be useful for the optimization of fast gradients. Due to ever increasing importance of short analysis times in modern HPLC practice, fast gradient elution is becoming widely used both in one-dimensional and two-dimensional separations, especially in the second dimension where the speed of separation is of primary importance. For method development and optimization, prediction of retention in gradient elution from the isocratic data is very important. This is principally possible using the well-established theory of gradient elution, especially for reversed-phase separations. Feasibility of these approaches has been proven in conventional liquid chromatography and commercial prediction software is available for this purpose. However, fast gradient chromatography employing short columns and gradient times in between 1 and 2 min or even less, impose additional stringent demands on the accuracy of prediction, due to rapid changes in mobile phase composition and increased role of extra-column contributions and instrumental dwell volume on the retention. In the present work, possibilities of prediction of the gradient elution times from the isocratic data measured only at two mobile phase concentrations were investigated in fast chromatography of alkylbenzenes, phenylurea and triazine pesticides on five columns differing in lengths: two monolithic ones and three packed with particles of different size. In the second dimension, gradient times are strongly restricted by the short cycle frequency. The effects of the flow-rate on the elution times, on the accuracy of their prediction and on peak capacity were studied under time constraint conditions. Gradient theory can be useful for the optimization of fast gradients.
dcterms:title
Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns
skos:prefLabel
Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns
skos:notation
RIV/00216275:25310/13:39896312!RIV14-GA0-25310___
n14:predkladatel
n21:orjk%3A25310
n4:aktivita
n5:P n5:I
n4:aktivity
I, P(GAP206/12/0398)
n4:cisloPeriodika
1
n4:dodaniDat
n15:2014
n4:domaciTvurceVysledku
n12:4487877 n12:6390218
n4:druhVysledku
n7:J
n4:duvernostUdaju
n11:S
n4:entitaPredkladatele
n10:predkladatel
n4:idSjednocenehoVysledku
97580
n4:idVysledku
RIV/00216275:25310/13:39896312
n4:jazykVysledku
n17:eng
n4:klicovaSlova
pesticides; peak capacity; prediction of retention; fast gradient elution
n4:klicoveSlovo
n6:peak%20capacity n6:prediction%20of%20retention n6:fast%20gradient%20elution n6:pesticides
n4:kodStatuVydavatele
NL - Nizozemsko
n4:kontrolniKodProRIV
[D9534B0AE5DA]
n4:nazevZdroje
Journal of Chromatography A
n4:obor
n9:CB
n4:pocetDomacichTvurcuVysledku
2
n4:pocetTvurcuVysledku
2
n4:projekt
n18:GAP206%2F12%2F0398
n4:rokUplatneniVysledku
n15:2013
n4:svazekPeriodika
1278
n4:tvurceVysledku
Vyňuchalová, Kateřina Jandera, Pavel
n4:wos
000315011200006
s:issn
0021-9673
s:numberOfPages
9
n16:doi
10.1016/j.chroma.2012.12.058
n20:organizacniJednotka
25310