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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F12%3A39895677%21RIV13-GA0-25310___
rdf:type
n18:Vysledek skos:Concept
dcterms:description
In-line coupled comprehensive HILICxRP systems should offer larger selectivity differences and better two-dimensional orthogonality than coupled RPxRP systems. However, this may not apply for all systems. The HILIC selectivity depends on the mix of selective polar and non-polar interactions with the functional groups, but also with the matrix of polar columns and depends on the sample type. We synthesized a new polar monolithic sulfobetaine polymethacrylate capillary column with excellent efficiency for low-molecular compounds. When used in the first, HILIC dimension coupled to core-shell or monolithic RP columns in the second dimension, this column provides much improved ortogonality for two-dimensional separations of phenolic and flavonoid compounds, in comparison to silica-bonded Diol, Polyethylene glycol or Zwitterionic columns. We investigated the performance of 11 short 5 cm and 3 cm columns for fast (1-2 min) gradient second-dimension separations. Band broadening or distortion may occur in directly coupled comprehensive HILICxRP systems, due to strong solvent-strength differences between the mobile phases used in the first and in the second dimension. To suppress this effect, low fraction volumes were collected from a 0.5 mm I.D. capillary monolithic sulfobetaine column at the flow-rate of a few microliters per min, coupled in-line with various core-shell columns operated at the maximum flow-rate. This setup with simultaneous gradient elution in the HILIC and in the RP dimension provided successful separation of natural antioxidants. In-line coupled comprehensive HILICxRP systems should offer larger selectivity differences and better two-dimensional orthogonality than coupled RPxRP systems. However, this may not apply for all systems. The HILIC selectivity depends on the mix of selective polar and non-polar interactions with the functional groups, but also with the matrix of polar columns and depends on the sample type. We synthesized a new polar monolithic sulfobetaine polymethacrylate capillary column with excellent efficiency for low-molecular compounds. When used in the first, HILIC dimension coupled to core-shell or monolithic RP columns in the second dimension, this column provides much improved ortogonality for two-dimensional separations of phenolic and flavonoid compounds, in comparison to silica-bonded Diol, Polyethylene glycol or Zwitterionic columns. We investigated the performance of 11 short 5 cm and 3 cm columns for fast (1-2 min) gradient second-dimension separations. Band broadening or distortion may occur in directly coupled comprehensive HILICxRP systems, due to strong solvent-strength differences between the mobile phases used in the first and in the second dimension. To suppress this effect, low fraction volumes were collected from a 0.5 mm I.D. capillary monolithic sulfobetaine column at the flow-rate of a few microliters per min, coupled in-line with various core-shell columns operated at the maximum flow-rate. This setup with simultaneous gradient elution in the HILIC and in the RP dimension provided successful separation of natural antioxidants.
dcterms:title
Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography
skos:prefLabel
Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography Optimization of Comprehensive Two-Dimensional Gradient Chromatography Coupling In-Line Hydrophilic Interaction and Reversed Phase Liquid Chromatography
skos:notation
RIV/00216275:25310/12:39895677!RIV13-GA0-25310___
n18:predkladatel
n19:orjk%3A25310
n3:aktivita
n13:S n13:P
n3:aktivity
P(GAP206/12/0398), S
n3:cisloPeriodika
December
n3:dodaniDat
n12:2013
n3:domaciTvurceVysledku
n4:4487877 n4:8169314 n4:6390218 n4:1878840 n4:9884947
n3:druhVysledku
n11:J
n3:duvernostUdaju
n8:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
156940
n3:idVysledku
RIV/00216275:25310/12:39895677
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Phenolic compounds.; Gradient elution; HILIC; Two-dimensional liquid chromatography
n3:klicoveSlovo
n14:Two-dimensional%20liquid%20chromatography n14:HILIC n14:Phenolic%20compounds. n14:Gradient%20elution
n3:kodStatuVydavatele
NL - Nizozemsko
n3:kontrolniKodProRIV
[CE662D441BF8]
n3:nazevZdroje
Journal of Chromatography A
n3:obor
n9:CB
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
5
n3:projekt
n15:GAP206%2F12%2F0398
n3:rokUplatneniVysledku
n12:2012
n3:svazekPeriodika
1268
n3:tvurceVysledku
Hájek, Tomáš Staňková, Magda Vyňuchalová, Kateřina Česla, Petr Jandera, Pavel
n3:wos
000312177300012
s:issn
0021-9673
s:numberOfPages
11
n16:doi
10.1016/j.chroma.2012.10.041
n20:organizacniJednotka
25310