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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F12%3A39894985%21RIV13-MSM-25310___
rdf:type
n6:Vysledek skos:Concept
dcterms:description
Cystic fibrosis is a serious genetic disorder affecting respiratory, gastrointestinal and genital tracts. The most common causes of health status deterioration of patients with cystic fibrosis are still considered to be bacterial infections. Staphylococcus aureus is one of the most common pathogens isolated from the air passages of these patients. The purpose of our study was to determine the ability and intensity of the biofilm production by 59 of Staphylococcus aureus strains isolated from the air passages of patients with cystic fibrosis in the Cystic fibrosis center at the University Hospital Hradec Kralové. We also evaluated biofilm production and its intensity in 59 Staphylococcus aureus strains isolated from the air passages of control group patients. The control group was formed by patients with nosocomial pneumonia hospitalized in the University Hospital. For assessing the biofilm production, we used modified microtiter-plate test by Christensen. We divided the intensity of biofilm production into four categories according to the measured optical density - negative, weak, medium and strong. Out of total 59 Staphylococcus aureus strains isolated from the air passages of cystic fibrosis patients 45 (77.2 %) strains produced biofilm in various degrees: 21 (35.6 %) weakly, 6 (10.2 %) medium and 18 (30.5 %) strongly. Similar results were determined for strains isolated from control group: 22 (37.3 %) weak, 13 (22.0 %) medium and 9 (15.3 %) strong biofilm producing strains. We found no statistically significant difference in the biofilm formation between both groups of Staphylococcus aureus strains. Cystic fibrosis is a serious genetic disorder affecting respiratory, gastrointestinal and genital tracts. The most common causes of health status deterioration of patients with cystic fibrosis are still considered to be bacterial infections. Staphylococcus aureus is one of the most common pathogens isolated from the air passages of these patients. The purpose of our study was to determine the ability and intensity of the biofilm production by 59 of Staphylococcus aureus strains isolated from the air passages of patients with cystic fibrosis in the Cystic fibrosis center at the University Hospital Hradec Kralové. We also evaluated biofilm production and its intensity in 59 Staphylococcus aureus strains isolated from the air passages of control group patients. The control group was formed by patients with nosocomial pneumonia hospitalized in the University Hospital. For assessing the biofilm production, we used modified microtiter-plate test by Christensen. We divided the intensity of biofilm production into four categories according to the measured optical density - negative, weak, medium and strong. Out of total 59 Staphylococcus aureus strains isolated from the air passages of cystic fibrosis patients 45 (77.2 %) strains produced biofilm in various degrees: 21 (35.6 %) weakly, 6 (10.2 %) medium and 18 (30.5 %) strongly. Similar results were determined for strains isolated from control group: 22 (37.3 %) weak, 13 (22.0 %) medium and 9 (15.3 %) strong biofilm producing strains. We found no statistically significant difference in the biofilm formation between both groups of Staphylococcus aureus strains.
dcterms:title
Biofilm production by Staphylococcus aureus strains isolated from cystic fibrosis patients Biofilm production by Staphylococcus aureus strains isolated from cystic fibrosis patients
skos:prefLabel
Biofilm production by Staphylococcus aureus strains isolated from cystic fibrosis patients Biofilm production by Staphylococcus aureus strains isolated from cystic fibrosis patients
skos:notation
RIV/00216275:25310/12:39894985!RIV13-MSM-25310___
n6:predkladatel
n18:orjk%3A25310
n3:aktivita
n4:S n4:I
n3:aktivity
I, S
n3:cisloPeriodika
prosinec
n3:dodaniDat
n14:2013
n3:domaciTvurceVysledku
n11:5566665 n11:3571726
n3:druhVysledku
n17:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n15:predkladatel
n3:idSjednocenehoVysledku
125036
n3:idVysledku
RIV/00216275:25310/12:39894985
n3:jazykVysledku
n16:eng
n3:klicovaSlova
biofilm; cystic fibrosis; Staphylococcus aureus
n3:klicoveSlovo
n10:Staphylococcus%20aureus n10:cystic%20fibrosis n10:biofilm
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[1CEA459B3B6A]
n3:nazevZdroje
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
n3:obor
n7:EE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n14:2012
n3:svazekPeriodika
18
n3:tvurceVysledku
Kukla, Rudolf Mazurová, Jaroslava Paterová, Pavla
s:issn
1211-5541
s:numberOfPages
10
n13:organizacniJednotka
25310