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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F12%3A39894651%21RIV13-MSM-25310___
rdf:type
skos:Concept n11:Vysledek
dcterms:description
Determination of amyloid beta (Abeta) isoforms - and in particular the proportion of the Abeta 1-42 isoform - in cerebrospinal fluid (CSF) of patients suspected of Alzheimer's disease (AD) might help in early diagnosis and treatment of that illness. Due to the low concentration of Abeta peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (mýIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Abeta antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the proper beads and anti-Abeta antibodies, as well as optimizing the immobilization technique and mýIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Abeta IS for mýIP and preconcentration of 5 Abeta peptides under optimized conditions using various subsequent analytical methods, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), capillary electrophoresis (CE), microchip electrophoresis (MCE), and immunoblotting. Synthetic Abeta peptides samples prepared in buffer and spiked in human CSF were analyzed. Finally, on-chip immunoprecipitation of Abeta peptides in human CSF sample was performed. Determination of amyloid beta (Abeta) isoforms - and in particular the proportion of the Abeta 1-42 isoform - in cerebrospinal fluid (CSF) of patients suspected of Alzheimer's disease (AD) might help in early diagnosis and treatment of that illness. Due to the low concentration of Abeta peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (mýIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Abeta antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the proper beads and anti-Abeta antibodies, as well as optimizing the immobilization technique and mýIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Abeta IS for mýIP and preconcentration of 5 Abeta peptides under optimized conditions using various subsequent analytical methods, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), capillary electrophoresis (CE), microchip electrophoresis (MCE), and immunoblotting. Synthetic Abeta peptides samples prepared in buffer and spiked in human CSF were analyzed. Finally, on-chip immunoprecipitation of Abeta peptides in human CSF sample was performed.
dcterms:title
Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers
skos:prefLabel
Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer's disease biomarkers
skos:notation
RIV/00216275:25310/12:39894651!RIV13-MSM-25310___
n11:predkladatel
n12:orjk%3A25310
n3:aktivita
n6:R
n3:aktivity
R
n3:cisloPeriodika
2
n3:dodaniDat
n17:2013
n3:domaciTvurceVysledku
n13:2568756 n13:9213627 n13:4136950
n3:druhVysledku
n4:J
n3:duvernostUdaju
n10:S
n3:entitaPredkladatele
n14:predkladatel
n3:idSjednocenehoVysledku
130746
n3:idVysledku
RIV/00216275:25310/12:39894651
n3:jazykVysledku
n19:eng
n3:klicovaSlova
biomarker; Alzheimer's disease; amyloid beta; preconcentration; microfluidics; immunosorbent
n3:klicoveSlovo
n9:biomarker n9:immunosorbent n9:preconcentration n9:amyloid%20beta n9:microfluidics n9:Alzheimer%27s%20disease
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[3DC1417CCCBD]
n3:nazevZdroje
Biomicrofluidics
n3:obor
n18:CB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
10
n3:rokUplatneniVysledku
n17:2012
n3:svazekPeriodika
6
n3:tvurceVysledku
Viovy, Jean-Louis Wiltfang, Jens Jankovičová, Barbora Verpillot, Romain Esselmann, Hermann Bílková, Zuzana Svobodová, Zuzana Otto, Markus Mohamadi, Mohamad Reza Taverna, Myriam
n3:wos
000305839800037
s:issn
1932-1058
s:numberOfPages
12
n16:doi
10.1063/1.4722588
n15:organizacniJednotka
25310