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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F11%3A39892617%21RIV12-MSM-25310___
rdf:type
skos:Concept n10:Vysledek
dcterms:description
Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization of the PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time used and tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further development and adaptation for flowing streams are also outlined. Conductive polymer polyaniline (PANI) was electrochemically synthesized by controlled potential cycling as a film at the surface of a carbon paste electrode (CPE) from acidic aqueous solution containing 1 M HCl and 0.25 M aniline. The characterization of the PANI-CPE was carried out with the aid of cyclic voltammetry in 1 M HCl, obtaining three couple of peaks from which the two pairs were distinct, having proved the electroactivity of the PANI film. Then, the PANI-CPE system was for the first time used and tested as glucose biosensor, when the respective enzyme was potentiostatically immobilized onto the surface from 0.1 M phosphate buffer (pH 7.5) with 0.75 mg/mL GOD. Optimal conditions for the enzyme immobilization were investigated and the biosensor tested for glucose sensing within the concentration range of 1-500 mg/L D-glucose. All preliminary assays with the GOD/PANI-CPE configuration have shown favorable operational features of this new carbon paste biosensor whose further development and adaptation for flowing streams are also outlined.
dcterms:title
A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film
skos:prefLabel
A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film A New Biosensor for Glucose Based on Carbon Paste and Enzyme Immobilized onto the Polyaniline Film
skos:notation
RIV/00216275:25310/11:39892617!RIV12-MSM-25310___
n10:predkladatel
n11:orjk%3A25310
n3:aktivita
n13:P n13:Z
n3:aktivity
P(LC06035), Z(MSM0021627502)
n3:cisloPeriodika
6
n3:dodaniDat
n5:2012
n3:domaciTvurceVysledku
n4:8608237 n4:6143083 n4:9805524
n3:druhVysledku
n9:J
n3:duvernostUdaju
n6:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
183934
n3:idVysledku
RIV/00216275:25310/11:39892617
n3:jazykVysledku
n14:eng
n3:klicovaSlova
Glucose; Glucose oxidase; Polyaniline film; Carbon paste; Biosensor
n3:klicoveSlovo
n15:Carbon%20paste n15:Biosensor n15:Glucose n15:Glucose%20oxidase n15:Polyaniline%20film
n3:kodStatuVydavatele
CS - Státní svaz Srbska a Černé Hory
n3:kontrolniKodProRIV
[3F69E73D32CF]
n3:nazevZdroje
International Journal of Electrochemical Science
n3:obor
n20:CG
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
4
n3:projekt
n16:LC06035
n3:rokUplatneniVysledku
n5:2011
n3:svazekPeriodika
6
n3:tvurceVysledku
Švancara, Ivan Vytřas, Karel Stočes, Matěj Kalcher, Kurt
n3:wos
000292330700014
n3:zamer
n19:MSM0021627502
s:issn
1452-3981
s:numberOfPages
10
n18:organizacniJednotka
25310