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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F11%3A39892302%21RIV12-MSM-25310___
rdf:type
n13:Vysledek skos:Concept
dcterms:description
We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested , inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant(R)Deoxynivalenol Test kit and Agra Quant(R) T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains was confirmed by PCR. The enzyme - trichodiene synthase - catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes. We examine the effects of selected fungicides on the Fusarium growth and toxins production especially of DON and T-2 toxins. Appropriate nutritive media were prepared containing different concentrations of the fungicides tested , inoculated with Fusarium moulds, and incubated under various conditions. The extraction of Fusarium toxins and their determination were carried out after the incubation using Agra Quant(R)Deoxynivalenol Test kit and Agra Quant(R) T-2 toxin Test kit. The results indicated a considerable variability of the individual strains in the formation of toxins. The strains of Fusarium graminearum CCM F-683 and Fusarium spp. produced large quantities of both DON and T-2 toxins. Fusarium poae CCM F-584 and Fusarium sporotrichioides CCM F-352 always produced larger quantities of only T-2 toxin in the given nutrient media. The experiment showed that the use of lower concentrations of fungicides resulted in an increased production of the monitored mycotoxins. The presence of Tri5 gene in the tested Fusarium strains was confirmed by PCR. The enzyme - trichodiene synthase - catalyses the isomerisation and cyclisation of farnesyl phosphate to trichodiene, the first step in the biosynthetic pathway of trichothecenes.
dcterms:title
Effect of Selected Fungicides on Fusarium Growth and toxins production Effect of Selected Fungicides on Fusarium Growth and toxins production
skos:prefLabel
Effect of Selected Fungicides on Fusarium Growth and toxins production Effect of Selected Fungicides on Fusarium Growth and toxins production
skos:notation
RIV/00216275:25310/11:39892302!RIV12-MSM-25310___
n13:predkladatel
n14:orjk%3A25310
n3:aktivita
n9:S n9:Z
n3:aktivity
S, Z(MSM0021627502)
n3:cisloPeriodika
SI
n3:dodaniDat
n18:2012
n3:domaciTvurceVysledku
n8:9591281 n8:3777111 n8:3491625
n3:druhVysledku
n4:J
n3:duvernostUdaju
n19:S
n3:entitaPredkladatele
n10:predkladatel
n3:idSjednocenehoVysledku
196443
n3:idVysledku
RIV/00216275:25310/11:39892302
n3:jazykVysledku
n11:eng
n3:klicovaSlova
PCR; fungicide; T-2 toxin; deoxynivalenol; mycotoxin; genus Fusarium
n3:klicoveSlovo
n7:deoxynivalenol n7:PCR n7:mycotoxin n7:fungicide n7:T-2%20toxin n7:genus%20Fusarium
n3:kodStatuVydavatele
CZ - Česká republika
n3:kontrolniKodProRIV
[19424C14E663]
n3:nazevZdroje
Czech journal of food sciences (Potravinářské vědy)
n3:obor
n16:EE
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:rokUplatneniVysledku
n18:2011
n3:svazekPeriodika
29
n3:tvurceVysledku
Hrubošová, Dana Vytřasová, Jarmila Moťková, Petra
n3:wos
000299721700011
n3:zamer
n12:MSM0021627502
s:issn
1212-1800
s:numberOfPages
7
n15:organizacniJednotka
25310