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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F10%3A39882327%21RIV11-GA0-25310___
rdf:type
skos:Concept n7:Vysledek
dcterms:description
A 2-D method was developed for separation of phenolic acids and flavone compounds combining LC with MEKC. The effect of substituted neutral and anionic CD additives to the background electrolyte on the quality of MEKC separation was investigated. The best selectivity of the MEKC separation was achieved in 25 mmol/L borate background buffer at pH 9.05 with the addition of 10 g/L SDS and 1.85 g/L heptakis (6-O-sulfo)-b-CD. These conditions were used in the second dimension of 2-D combination of LC and MEKC separation in combination with a PEG column in the first dimension, providing the best orthogonality (the lowest degree of correlation between the selectivity of separation) in the two dimensions. A CE autosampler was employed as the interface between LC and MEKC steps based on automated fraction collection before the re-analysis of the collected LC fractions in the second, MEKC dimension. A 2-D method was developed for separation of phenolic acids and flavone compounds combining LC with MEKC. The effect of substituted neutral and anionic CD additives to the background electrolyte on the quality of MEKC separation was investigated. The best selectivity of the MEKC separation was achieved in 25 mmol/L borate background buffer at pH 9.05 with the addition of 10 g/L SDS and 1.85 g/L heptakis (6-O-sulfo)-b-CD. These conditions were used in the second dimension of 2-D combination of LC and MEKC separation in combination with a PEG column in the first dimension, providing the best orthogonality (the lowest degree of correlation between the selectivity of separation) in the two dimensions. A CE autosampler was employed as the interface between LC and MEKC steps based on automated fraction collection before the re-analysis of the collected LC fractions in the second, MEKC dimension.
dcterms:title
Separation of Phenolic Acids and Flavone Natural Antioxidants by Two-Dimensional Method Combining Liquid Chromatography and Micellar Electrokinetic Capillary Chromatography Separation of Phenolic Acids and Flavone Natural Antioxidants by Two-Dimensional Method Combining Liquid Chromatography and Micellar Electrokinetic Capillary Chromatography
skos:prefLabel
Separation of Phenolic Acids and Flavone Natural Antioxidants by Two-Dimensional Method Combining Liquid Chromatography and Micellar Electrokinetic Capillary Chromatography Separation of Phenolic Acids and Flavone Natural Antioxidants by Two-Dimensional Method Combining Liquid Chromatography and Micellar Electrokinetic Capillary Chromatography
skos:notation
RIV/00216275:25310/10:39882327!RIV11-GA0-25310___
n3:aktivita
n14:Z n14:P
n3:aktivity
P(GA203/07/0641), P(GP203/09/P199), Z(MSM0021627502)
n3:cisloPeriodika
13
n3:dodaniDat
n10:2011
n3:domaciTvurceVysledku
n4:4487877 n4:2967391 n4:8169314
n3:druhVysledku
n16:J
n3:duvernostUdaju
n13:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
286996
n3:idVysledku
RIV/00216275:25310/10:39882327
n3:jazykVysledku
n19:eng
n3:klicovaSlova
Phenolic compounds; MEKC; Flavones; 2-D separations
n3:klicoveSlovo
n6:2-D%20separations n6:MEKC n6:Phenolic%20compounds n6:Flavones
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[62F124496271]
n3:nazevZdroje
Electrophoresis
n3:obor
n15:CB
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
3
n3:projekt
n12:GA203%2F07%2F0641 n12:GP203%2F09%2FP199
n3:rokUplatneniVysledku
n10:2010
n3:svazekPeriodika
31
n3:tvurceVysledku
Fischer, Jan Česla, Petr Jandera, Pavel
n3:wos
000280226900013
n3:zamer
n17:MSM0021627502
s:issn
0173-0835
s:numberOfPages
11
n8:organizacniJednotka
25310