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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F10%3A39881939%21RIV11-GA0-25310___
rdf:type
skos:Concept n16:Vysledek
dcterms:description
The analytical method based on the high-per formance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin,5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL-1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43 % of DPPH radical inhibition for PFE method and from 76.01 to 89.94 % in the case of USE method. The analytical method based on the high-per formance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin,5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL-1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43 % of DPPH radical inhibition for PFE method and from 76.01 to 89.94 % in the case of USE method.
dcterms:title
Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity
skos:prefLabel
Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity Comparison of Pressurised Fluid and Ultrasonic Extraction Methods for Analysis of Plant Antioxidants and their Antioxidant Capacity
skos:notation
RIV/00216275:25310/10:39881939!RIV11-GA0-25310___
n3:aktivita
n7:Z n7:P
n3:aktivity
P(GA203/08/1536), Z(MSM0021627502)
n3:cisloPeriodika
1
n3:dodaniDat
n5:2011
n3:domaciTvurceVysledku
n8:3933164 n8:5849535 n8:6505961 n8:2804417 n8:3799794 n8:8088462
n3:druhVysledku
n13:J
n3:duvernostUdaju
n4:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
251502
n3:idVysledku
RIV/00216275:25310/10:39881939
n3:jazykVysledku
n14:eng
n3:klicovaSlova
High-Performance Liquid Chromatography; Antioxidant Capacity; Antioxidants; Ultrasonic Extraction; Pressurised Fluid Extraction
n3:klicoveSlovo
n12:Antioxidant%20Capacity n12:High-Performance%20Liquid%20Chromatography n12:Antioxidants n12:Pressurised%20Fluid%20Extraction n12:Ultrasonic%20Extraction
n3:kodStatuVydavatele
PL - Polská republika
n3:kontrolniKodProRIV
[726DC51E2C08]
n3:nazevZdroje
Central European Journal of Chemistry
n3:obor
n19:CB
n3:pocetDomacichTvurcuVysledku
6
n3:pocetTvurcuVysledku
6
n3:projekt
n15:GA203%2F08%2F1536
n3:rokUplatneniVysledku
n5:2010
n3:svazekPeriodika
8
n3:tvurceVysledku
Dobiáš, Petr Beňová, Blanka Eisner, Aleš Pavlíková, Petra Ventura, Karel Adam, Martin
n3:wos
000276548100012
n3:zamer
n6:MSM0021627502
s:issn
1895-1066
s:numberOfPages
9
n18:organizacniJednotka
25310