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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F10%3A39881444%21RIV13-GA0-25310___
rdf:type
skos:Concept n18:Vysledek
dcterms:description
An electrochemical biosensor for specific detection of DNA sequences from Aspergillus, a potentially aflatoxigenic fungus is reported. The detection technique was optimized using short DNA sequences, then applied for a 92-mer from the Aspergillus gene aflR that codes a biochemical pathway of aflatoxin B1 production. Voltammetric detection of the specific Aspergillus DNA sequence is based on hybridization of adsorbed target DNA with a biotinylated probe and subsequent binding with streptavidin alkaline phosphatase conjugate. Such modified electrode surface of carbon paste electrode (CPE) is incubated in a buffer with an electrochemically inactive substrate (1-naphthyl phosphate). Alkaline phosphatase converts 1-naphthyl phosphate into 1-naphthol, which is determined by the selected voltammetric technique. The optimized procedure provided good differentiation between complementary and noncomplementary probes and also between potentially aflatoxigenic and non aflatoxigenic fungi of the genus Aspergillus. An electrochemical biosensor for specific detection of DNA sequences from Aspergillus, a potentially aflatoxigenic fungus is reported. The detection technique was optimized using short DNA sequences, then applied for a 92-mer from the Aspergillus gene aflR that codes a biochemical pathway of aflatoxin B1 production. Voltammetric detection of the specific Aspergillus DNA sequence is based on hybridization of adsorbed target DNA with a biotinylated probe and subsequent binding with streptavidin alkaline phosphatase conjugate. Such modified electrode surface of carbon paste electrode (CPE) is incubated in a buffer with an electrochemically inactive substrate (1-naphthyl phosphate). Alkaline phosphatase converts 1-naphthyl phosphate into 1-naphthol, which is determined by the selected voltammetric technique. The optimized procedure provided good differentiation between complementary and noncomplementary probes and also between potentially aflatoxigenic and non aflatoxigenic fungi of the genus Aspergillus.
dcterms:title
Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species
skos:prefLabel
Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species Carbon paste electrode for voltammetric detection of a specific DNA sequence from potentially aflatoxigenic Aspergillus species
skos:notation
RIV/00216275:25310/10:39881444!RIV13-GA0-25310___
n3:aktivita
n16:Z n16:P
n3:aktivity
P(GA203/09/0148), P(LC06035), Z(MSM0021627502)
n3:cisloPeriodika
1
n3:dodaniDat
n13:2013
n3:domaciTvurceVysledku
n7:9403515 n7:6143083 n7:3777111 n7:9465421 n7:3491625
n3:druhVysledku
n19:J
n3:duvernostUdaju
n12:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
249613
n3:idVysledku
RIV/00216275:25310/10:39881444
n3:jazykVysledku
n4:eng
n3:klicovaSlova
voltammetry; carbon paste electrode; DNA hybridization; Aspergillus fungi
n3:klicoveSlovo
n6:DNA%20hybridization n6:carbon%20paste%20electrode n6:Aspergillus%20fungi n6:voltammetry
n3:kodStatuVydavatele
US - Spojené státy americké
n3:kontrolniKodProRIV
[63DF7A1F9C3E]
n3:nazevZdroje
Electrochemistry Communications
n3:obor
n14:CG
n3:pocetDomacichTvurcuVysledku
5
n3:pocetTvurcuVysledku
6
n3:projekt
n11:GA203%2F09%2F0148 n11:LC06035
n3:rokUplatneniVysledku
n13:2010
n3:svazekPeriodika
12
n3:tvurceVysledku
Vytřas, Karel Tisoň, Lukáš Šnévajsová, Petra Metelka, Radovan Vytřasová, Jarmila Brožková, Iveta
n3:wos
000274233700026
n3:zamer
n17:MSM0021627502
s:issn
1388-2481
s:numberOfPages
4
n20:doi
10.1016/j.elecom.2009.10.047
n15:organizacniJednotka
25310