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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F09%3A00009576%21RIV10-MSM-25310___
rdf:type
n8:Vysledek skos:Concept
dcterms:description
The title 5-substituted pyrimidines (U and C) and 7-substituted 7-deazapurines (7-deazaA and 7-deazaG) bearing tetrathiafulvelene (TTF) attached through an acetylene linker have been prepared by cross-coupling of the corresponding 5- or 7-iodo derivatives of nucleosides with 2-ethynyltetrathiafulvalene. Their subsequent triphosphorylation gave the corresponding nucleoside triphosphates (dNTPs). Square-wave voltammetry of the TTF-labelled nucleosides and nucleotides showed two peaks, which correspond to two reversible one-electron redox processes in the TTF moiety. Polymerase incorporation of the TTF-labelled dNTPs into DNA has also been studied. Multiple incorporations were rather problematic and only by using dCTTFTP was efficient primer extension observed with Vent (exo-)polymerase. Single nucleotide extension was successful with labelled A (dA*TTFTP) and C (dCTTFTP) nucleotides. Inhibition of the polymerase was observed at higher concentrations of dNTTFTPs. The title 5-substituted pyrimidines (U and C) and 7-substituted 7-deazapurines (7-deazaA and 7-deazaG) bearing tetrathiafulvelene (TTF) attached through an acetylene linker have been prepared by cross-coupling of the corresponding 5- or 7-iodo derivatives of nucleosides with 2-ethynyltetrathiafulvalene. Their subsequent triphosphorylation gave the corresponding nucleoside triphosphates (dNTPs). Square-wave voltammetry of the TTF-labelled nucleosides and nucleotides showed two peaks, which correspond to two reversible one-electron redox processes in the TTF moiety. Polymerase incorporation of the TTF-labelled dNTPs into DNA has also been studied. Multiple incorporations were rather problematic and only by using dCTTFTP was efficient primer extension observed with Vent (exo-)polymerase. Single nucleotide extension was successful with labelled A (dA*TTFTP) and C (dCTTFTP) nucleotides. Inhibition of the polymerase was observed at higher concentrations of dNTTFTPs.
dcterms:title
Tetrathiafulvalene-Labelled Nucleosides and Nucleoside Triphosphates: Synthesis, Electrochemistry and the Scope of Their Polymerase Incorporation into DNA Tetrathiafulvalene-Labelled Nucleosides and Nucleoside Triphosphates: Synthesis, Electrochemistry and the Scope of Their Polymerase Incorporation into DNA
skos:prefLabel
Tetrathiafulvalene-Labelled Nucleosides and Nucleoside Triphosphates: Synthesis, Electrochemistry and the Scope of Their Polymerase Incorporation into DNA Tetrathiafulvalene-Labelled Nucleosides and Nucleoside Triphosphates: Synthesis, Electrochemistry and the Scope of Their Polymerase Incorporation into DNA
skos:notation
RIV/00216275:25310/09:00009576!RIV10-MSM-25310___
n3:aktivita
n18:P
n3:aktivity
P(LC06035)
n3:cisloPeriodika
21
n3:dodaniDat
n4:2010
n3:domaciTvurceVysledku
n17:6875181
n3:druhVysledku
n16:J
n3:duvernostUdaju
n15:S
n3:entitaPredkladatele
n9:predkladatel
n3:idSjednocenehoVysledku
346099
n3:idVysledku
RIV/00216275:25310/09:00009576
n3:jazykVysledku
n6:eng
n3:klicovaSlova
nucleosides; nucleotides; nucleic acids; cross-coupling; DNA polymerase; tetrathiafulvalene
n3:klicoveSlovo
n7:cross-coupling n7:nucleic%20acids n7:nucleotides n7:tetrathiafulvalene n7:DNA%20polymerase n7:nucleosides
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[C8F5754B691C]
n3:nazevZdroje
European Journal of Organic Chemistry
n3:obor
n10:CG
n3:pocetDomacichTvurcuVysledku
1
n3:pocetTvurcuVysledku
7
n3:projekt
n12:LC06035
n3:rokUplatneniVysledku
n4:2009
n3:svazekPeriodika
2009
n3:tvurceVysledku
Fojta, Miroslav Pohl, Radek Horáková, Petra Hocek, Michal Riedl, Jan Havran, Luděk Šebest, Peter
s:issn
1434-193X
s:numberOfPages
7
n14:organizacniJednotka
25310