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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F07%3A00006415%21RIV08-MSM-25310___
rdf:type
skos:Concept n21:Vysledek
dcterms:description
Amperometrická detekce Salmonella Enteritidis s použitím uhlíkové pastové elektrody An amperometric method for samonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0,65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella enteritidis in a sample. An amperometric method for samonella detection was optimized. The method is based on the reaction of salmonella with an enzyme-linked specific antibody (alkaline phosphatase), forming salmonella-antibody-alkaline phosphatase (SAAP) conjugates. After their hydrolysis, the products formed (phenols) are detected amperometrically using a carbon paste electrode; current signals were monitored at 0,65 V vs. Ag/AgCl/sat. KCl. A medium of pH = 10 was found as the best. Amount of phenol generated by SAAP was proportional to the number of Salmonella enteritidis in a sample.
dcterms:title
A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes Amperometrická detekce Salmonella Enteritidis s použitím uhlíkové pastové elektrody
skos:prefLabel
Amperometrická detekce Salmonella Enteritidis s použitím uhlíkové pastové elektrody A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes A Study of the Amperometric Method for Salmonella Enteritidis Detection Using Carbon Paste Electrodes
skos:notation
RIV/00216275:25310/07:00006415!RIV08-MSM-25310___
n3:strany
201-208
n3:aktivita
n4:P n4:Z
n3:aktivity
P(LC06035), Z(MSM0021627502)
n3:dodaniDat
n15:2008
n3:domaciTvurceVysledku
n6:7862105 n6:3777111 n6:6143083
n3:druhVysledku
n8:C
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n12:predkladatel
n3:idSjednocenehoVysledku
408229
n3:idVysledku
RIV/00216275:25310/07:00006415
n3:jazykVysledku
n13:eng
n3:klicovaSlova
Salmonella enteritidis; carbon paste electrode; amperometric detection; alkaline phosphatase.
n3:klicoveSlovo
n10:amperometric%20detection n10:Salmonella%20enteritidis n10:carbon%20paste%20electrode n10:alkaline%20phosphatase.
n3:kontrolniKodProRIV
[C5B4AFEB45C3]
n3:mistoVydani
Pardubice
n3:nazevZdroje
Sensing in Electroanalysis
n3:obor
n9:CG
n3:pocetDomacichTvurcuVysledku
3
n3:pocetTvurcuVysledku
5
n3:projekt
n16:LC06035
n3:rokUplatneniVysledku
n15:2007
n3:tvurceVysledku
Hrubeš, Michal Pešková, Iva Vytřas, Karel Červenka, Libor Vytřasová, Jarmila
n3:zamer
n17:MSM0021627502
s:numberOfPages
8
n19:hasPublisher
Univerzita Pardubice
n20:isbn
978-80-7194-954-1
n11:organizacniJednotka
25310