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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F06%3A00004342%21RIV08-MSM-25310___
rdf:type
n5:Vysledek skos:Concept
dcterms:description
The preparation of an easily replaceable protease microreactor for micro-chip application is described. Magnetic particles coated with poly(N-isopropylacrylamide), polystyrene, poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate), poly(glycidyl methacrylate), [(2-amino-ethyl)hydroxymethylen]biphosphonic acid, or alginic acid with immobilized trypsin were utilized for heterogeneous digestion. The properties were optimized, with the constraint of allowing immobilization in a microchannel by a magnetic field gradient. To obtain the highest digestion efficiency, sub-micrometer spheres were organized by an inhomogeneous external magnetic field perpendicularly to the direction of the channel. Kinetic parameters of the enzyme reactor immobilized in micro-chip capillary (micro-chip immobilized magnetic enzyme reactor (IMER)) were determined. The capability of the proteolytic reactor was demonstrated by five model (glyco)proteins ranging in molecular mass from 4.3 to 150 kDa. Digestion efficiency of pr The preparation of an easily replaceable protease microreactor for micro-chip application is described. Magnetic particles coated with poly(N-isopropylacrylamide), polystyrene, poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate), poly(glycidyl methacrylate), [(2-amino-ethyl)hydroxymethylen]biphosphonic acid, or alginic acid with immobilized trypsin were utilized for heterogeneous digestion. The properties were optimized, with the constraint of allowing immobilization in a microchannel by a magnetic field gradient. To obtain the highest digestion efficiency, sub-micrometer spheres were organized by an inhomogeneous external magnetic field perpendicularly to the direction of the channel. Kinetic parameters of the enzyme reactor immobilized in micro-chip capillary (micro-chip immobilized magnetic enzyme reactor (IMER)) were determined. The capability of the proteolytic reactor was demonstrated by five model (glyco)proteins ranging in molecular mass from 4.3 to 150 kDa. Digestion efficiency of pr The preparation of an easily replaceable protease microreactor for micro-chip application is described. Magnetic particles coated with poly(N-isopropylacrylamide), polystyrene, poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate), poly(glycidyl methacrylate), [(2-amino-ethyl)hydroxymethylen]biphosphonic acid, or alginic acid with immobilized trypsin were utilized for heterogeneous digestion. The properties were optimized, with the constraint of allowing immobilization in a microchannel by a magnetic field gradient. To obtain the highest digestion efficiency, sub-micrometer spheres were organized by an inhomogeneous external magnetic field perpendicularly to the direction of the channel. Kinetic parameters of the enzyme reactor immobilized in micro-chip capillary (micro-chip immobilized magnetic enzyme reactor (IMER)) were determined. The capability of the proteolytic reactor was demonstrated by five model (glyco)proteins ranging in molecular mass from 4.3 to 150 kDa. Digestion efficiency of pr
dcterms:title
Functionalized magnetic micro- and nanoparticles: optimization and application to ?-chip trypsin digestion Functionalized magnetic micro- and nanoparticles: optimization and application to ?-chip trypsin digestion Functionalized magnetic micro- and nanoparticles: optimization and application to ?-chip trypsin digestion
skos:prefLabel
Functionalized magnetic micro- and nanoparticles: optimization and application to ?-chip trypsin digestion Functionalized magnetic micro- and nanoparticles: optimization and application to ?-chip trypsin digestion Functionalized magnetic micro- and nanoparticles: optimization and application to ?-chip trypsin digestion
skos:notation
RIV/00216275:25310/06:00004342!RIV08-MSM-25310___
n3:strany
811-24
n3:aktivita
n12:P n12:Z
n3:aktivity
P(GA203/02/0023), P(GA203/05/0241), Z(AV0Z40310501), Z(AV0Z40500505), Z(MSM0021627502)
n3:cisloPeriodika
27
n3:dodaniDat
n4:2008
n3:domaciTvurceVysledku
n16:5793181 n16:9213627
n3:druhVysledku
n7:J
n3:duvernostUdaju
n18:S
n3:entitaPredkladatele
n17:predkladatel
n3:idSjednocenehoVysledku
476478
n3:idVysledku
RIV/00216275:25310/06:00004342
n3:jazykVysledku
n13:eng
n3:klicovaSlova
magnetic particles; microCHIP magnetic resonance reactor; peptide; trypsin
n3:klicoveSlovo
n11:magnetic%20particles n11:trypsin n11:microCHIP%20magnetic%20resonance%20reactor n11:peptide
n3:kodStatuVydavatele
DE - Spolková republika Německo
n3:kontrolniKodProRIV
[BDE87E820AE8]
n3:nazevZdroje
Electrophoresis
n3:obor
n19:CE
n3:pocetDomacichTvurcuVysledku
2
n3:pocetTvurcuVysledku
11
n3:projekt
n14:GA203%2F02%2F0023 n14:GA203%2F05%2F0241
n3:rokUplatneniVysledku
n4:2006
n3:svazekPeriodika
2006
n3:tvurceVysledku
Fuetterer, Claus Poitier, Isabelle Horák, Daniel Minc, Nicolas Cecal, Roxana Przybylski, Michael Křenková, Jana Beneš, Milan Slováková, Marcela Viovy, Jean-Louis Bílková, Zuzana
n3:zamer
n15:AV0Z40500505 n15:AV0Z40310501 n15:MSM0021627502
s:issn
0173-0835
s:numberOfPages
14
n6:organizacniJednotka
25310