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Statements

Subject Item
n2:RIV%2F00216275%3A25310%2F06%3A00004294%21RIV08-MSM-25310___
rdf:type
skos:Concept n21:Vysledek
dcterms:description
určeno pro seznam publikací Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae and F. sporotrichioides is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals. Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food an Contamination of small-grain cereals with the fungal species Fusarium graminearum, F. culmorum, F. poae and F. sporotrichioides is an important source of trichothecenes, zearalenone and other mycotoxins which cause serious diseases in human and animals. Early detection and control of these Fusarium species is crucial to prevent toxins entering the food chain and a useful tool in disease management practices. Two PCR methods were evaluated for the successful identification of Fusarium species. The PCR was performed using a primer specifically targeted to Tri5 gene. The extraction technique of DNA by Cenis (1992) and the PCR procedure according to Edwards et al. (2001)) were compared with the DNA isolation and amplification using the Plant PCR Kit. The sensitivity of the latter method was determined using the reference strain of Fusarium graminearum CCM F-683 and the selectivity was examined using reference cultures of different genera of fungi naturally occurring in food and feedstuff. Fifty food an
dcterms:title
Toxinogenní fusária v potravinách a krmivech. Toxigenic Fusarium in Food and Feed Toxigenic Fusarium in Food and Feed
skos:prefLabel
Toxinogenní fusária v potravinách a krmivech. Toxigenic Fusarium in Food and Feed Toxigenic Fusarium in Food and Feed
skos:notation
RIV/00216275:25310/06:00004294!RIV08-MSM-25310___
n3:strany
412
n3:aktivita
n5:P n5:Z
n3:aktivity
P(GA203/05/2106), Z(MSM0021627502)
n3:dodaniDat
n7:2008
n3:domaciTvurceVysledku
n10:7862105 n10:7035411 n10:9403515 n10:3777111
n3:druhVysledku
n18:D
n3:duvernostUdaju
n11:S
n3:entitaPredkladatele
n19:predkladatel
n3:idSjednocenehoVysledku
504083
n3:idVysledku
RIV/00216275:25310/06:00004294
n3:jazykVysledku
n17:eng
n3:klicovaSlova
Fusarium spp.; Fusarium graminearum; toxigenic fungi; polymerase chain reaction; Tri5 gene; trichothecenes; mycotoxins
n3:klicoveSlovo
n4:trichothecenes n4:Tri5%20gene n4:Fusarium%20graminearum n4:toxigenic%20fungi n4:polymerase%20chain%20reaction n4:mycotoxins n4:Fusarium%20spp.
n3:kontrolniKodProRIV
[36B94DA8D0F9]
n3:mistoKonaniAkce
Bologna
n3:mistoVydani
Bologna, Itálie
n3:nazevZdroje
20th International ICFMH Symposium Food Micro 2006
n3:obor
n20:EE
n3:pocetDomacichTvurcuVysledku
4
n3:pocetTvurcuVysledku
5
n3:projekt
n14:GA203%2F05%2F2106
n3:rokUplatneniVysledku
n7:2006
n3:tvurceVysledku
Vytřasová, Jarmila Červenka, Libor Svobodová, Lucie Brožková, Iveta Pejchalová, Marcela
n3:typAkce
n22:WRD
n3:zahajeniAkce
2006-08-29+02:00
n3:zamer
n13:MSM0021627502
s:numberOfPages
1
n8:hasPublisher
International Committee on Food Microbiology and Hygiene
n16:isbn
961-90346-3-5
n6:organizacniJednotka
25310